Biofunctionalized AlGaN/GaN high electron mobility transistor for DNA hybridization detection

Resham Thapa; Siddharth Alur; Kyusang Kim; Fei Tong; Yogesh Sharma; Moonil Kim; Claude Ahyi; Jing Dai; Jong Wook Hong; Michael Bozack; John Williams; Ahjeong Son; Amir Dabiran; Minseo Park

doi:10.1063/1.4727895

Biofunctionalized AlGaN/GaN high electron mobility transistor for DNA hybridization detection

Resham Thapa, Siddharth Alur, Kyusang Kim, Fei Tong, Yogesh Sharma, Moonil Kim, Claude Ahyi, Jing Dai, Jong Wook Hong, Michael Bozack, John Williams, Ahjeong Son, Amir Dabiran, Minseo Park

Department of Environmental Science & Engineering

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Label-free electrical detection of deoxyribonucleic acid (DNA) hybridization was demonstrated using an AlGaN/GaN high electron mobility transistor (HEMT) based transducer with a biofunctionalized gate. The HEMT DNA sensor employed the immobilization of amine-modified single strand DNA on the self-assembled monolayers of 11-mercaptoundecanoic acid. The sensor exhibited a substantial current drop upon introduction of complimentary DNA to the gate well, which is a clear indication of the hybridization. The application of 3 base-pair mismatched target DNA showed little change in output current characteristics of the transistor. Therefore, it can be concluded that our DNA sensor is highly specific to DNA sequences.

Original language	English
Article number	232109
Journal	Applied Physics Letters
Volume	100
Issue number	23
DOIs	https://doi.org/10.1063/1.4727895
State	Published - 4 Jun 2012

Bibliographical note

Funding Information:
This project was funded by the United States Department of Agriculture (USDA) through Auburn University Detection and Food Safety Center (AUDFS). The authors would like to thank Dr. David Deen, SVT Associates, Inc., for his valuable comments to the manuscript and Dr. David Stanbury, Department of Chemistry and Biochemistry, Auburn University, for allowing us to use his UV-Vis spectrometer.

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title = "Biofunctionalized AlGaN/GaN high electron mobility transistor for DNA hybridization detection",

abstract = "Label-free electrical detection of deoxyribonucleic acid (DNA) hybridization was demonstrated using an AlGaN/GaN high electron mobility transistor (HEMT) based transducer with a biofunctionalized gate. The HEMT DNA sensor employed the immobilization of amine-modified single strand DNA on the self-assembled monolayers of 11-mercaptoundecanoic acid. The sensor exhibited a substantial current drop upon introduction of complimentary DNA to the gate well, which is a clear indication of the hybridization. The application of 3 base-pair mismatched target DNA showed little change in output current characteristics of the transistor. Therefore, it can be concluded that our DNA sensor is highly specific to DNA sequences.",

author = "Resham Thapa and Siddharth Alur and Kyusang Kim and Fei Tong and Yogesh Sharma and Moonil Kim and Claude Ahyi and Jing Dai and {Wook Hong}, Jong and Michael Bozack and John Williams and Ahjeong Son and Amir Dabiran and Minseo Park",

note = "Funding Information: This project was funded by the United States Department of Agriculture (USDA) through Auburn University Detection and Food Safety Center (AUDFS). The authors would like to thank Dr. David Deen, SVT Associates, Inc., for his valuable comments to the manuscript and Dr. David Stanbury, Department of Chemistry and Biochemistry, Auburn University, for allowing us to use his UV-Vis spectrometer. ",

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doi = "10.1063/1.4727895",

language = "English",

volume = "100",

journal = "Applied Physics Letters",

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T1 - Biofunctionalized AlGaN/GaN high electron mobility transistor for DNA hybridization detection

AU - Thapa, Resham

AU - Alur, Siddharth

AU - Kim, Kyusang

AU - Tong, Fei

AU - Sharma, Yogesh

AU - Kim, Moonil

AU - Ahyi, Claude

AU - Dai, Jing

AU - Wook Hong, Jong

AU - Bozack, Michael

AU - Williams, John

AU - Son, Ahjeong

AU - Dabiran, Amir

AU - Park, Minseo

N1 - Funding Information: This project was funded by the United States Department of Agriculture (USDA) through Auburn University Detection and Food Safety Center (AUDFS). The authors would like to thank Dr. David Deen, SVT Associates, Inc., for his valuable comments to the manuscript and Dr. David Stanbury, Department of Chemistry and Biochemistry, Auburn University, for allowing us to use his UV-Vis spectrometer.

PY - 2012/6/4

Y1 - 2012/6/4

N2 - Label-free electrical detection of deoxyribonucleic acid (DNA) hybridization was demonstrated using an AlGaN/GaN high electron mobility transistor (HEMT) based transducer with a biofunctionalized gate. The HEMT DNA sensor employed the immobilization of amine-modified single strand DNA on the self-assembled monolayers of 11-mercaptoundecanoic acid. The sensor exhibited a substantial current drop upon introduction of complimentary DNA to the gate well, which is a clear indication of the hybridization. The application of 3 base-pair mismatched target DNA showed little change in output current characteristics of the transistor. Therefore, it can be concluded that our DNA sensor is highly specific to DNA sequences.

AB - Label-free electrical detection of deoxyribonucleic acid (DNA) hybridization was demonstrated using an AlGaN/GaN high electron mobility transistor (HEMT) based transducer with a biofunctionalized gate. The HEMT DNA sensor employed the immobilization of amine-modified single strand DNA on the self-assembled monolayers of 11-mercaptoundecanoic acid. The sensor exhibited a substantial current drop upon introduction of complimentary DNA to the gate well, which is a clear indication of the hybridization. The application of 3 base-pair mismatched target DNA showed little change in output current characteristics of the transistor. Therefore, it can be concluded that our DNA sensor is highly specific to DNA sequences.

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VL - 100

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 23

M1 - 232109

ER -

Biofunctionalized AlGaN/GaN high electron mobility transistor for DNA hybridization detection

Abstract

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