Bioelectronic nose with high sensitivity and selectivity using chemically functionalized carbon nanotube combined with human olfactory receptor

Sang Hun Lee, Hye Jun Jin, Hyun Seok Song, Seunghun Hong, Tai Hyun Park

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

Single-walled carbon nanotubes (swCNTs) hold great promise for use as molecular wires because they exhibit high electrical conductivity and chemical stability. However, constructing swCNT-based transducer devices requires controlled strategies for assembling biomolecules on swCNTs. In this study, we proposed a chemically modified swCNT. The swCNT was functionalized with 1,5-diaminonaphthalene via π-stacking, for reliable attachment of the human olfactory receptor 2AG1 (hOR2AG1). The human olfactory receptor was then anchored. We investigated the use of this functionalized CNT in the fabrication of a highly sensitive and selective bioelectronic nose. For the bioelectronic nose, the swCNT-field effect transistor (FET) platform was composed of polyethylene glycol (PEG)-coated regions to prevent non-specific absorption and chemically modified swCNTs regions containing hOR2AG1, which can bind to the specific odorant. This approach allowed us to create well-defined micron-scale patterns of hOR2AG1 on the swCNTs. Our bioelectronic nose displayed ultrahigh sensitivity down to concentrations as low as 1. fM due to the enhanced hOR2AG1-odorant interaction through the tight binding of hOR2AG1 on the chemically modified swCNTs. In addition, the approach described here may provide an alternative route for multiplexed detection of diverse odorants and to improve the sensitivity of sensor devices.

Original languageEnglish
Pages (from-to)467-472
Number of pages6
JournalJournal of Biotechnology
Volume157
Issue number4
DOIs
StatePublished - 20 Feb 2012

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Ministry of Education, Science and Technology (MEST) (No. 2011-0000331 , No. 2011K000682 , No. 20100020821 , WCU project R32-2010-000-10213-0). SH acknowledges the support from the NRF grant (No. 2011-0000390 ) and in part from the WCU program (No. R31-10032 ). Also, this work was supported by the Converging Research Center Program (No. 2011K000683 ).

Keywords

  • Bioelectronic nose
  • Carbon nanotube
  • Field effect transistor
  • Functionalization
  • Human olfactory receptor

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