Bioelectronic nose using olfactory receptor-embedded nanodiscs

Heehong Yang, Minju Lee, Daesan Kim, Seunghun Hong, Tai Hyun Park

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Scopus citations


Olfactory receptors (ORs) are the largest family of the G protein-coupled receptors (GPCRs), which are significantly involved in many human diseases and 40% of all drug targets. A platform containing stable and high-quality OR would be a powerful tool for the development of a practical biosensor that can be applied to various applications, such as the early diagnosis of diseases, assessment of food quality, and drug and fragrance development. Significant efforts have been made to develop the biosensor using GPCRs; nevertheless, they remain a challenge. This chapter describes an attractive methodology for the development of a stable bioelectronic nose using OR-embedded nanodiscs. The ORs were produced in Escherichia coli (E. coli), purified with column chromatography, reconstituted into nanodiscs and applied to a carbon nanotube-field effect transistor (CNT-FET) with floating electrodes. The nanodisc-based bioelectronic nose exhibits high-performance in terms of sensitivity, selectivity and stability. This strategy can be used as a practical method for the receptor-based sensing approach, which represents significant progress in nano-bio technology toward a practical biosensor.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages11
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2018.


  • Bioelectronic nose
  • Carbon nanotube
  • Field-effect transistor
  • Nanodisc
  • Olfactory receptor


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