Bioelectronic nose using olfactory receptor-embedded nanodiscs

Heehong Yang; Minju Lee; Daesan Kim; Seunghun Hong; Tai Hyun Park

doi:10.1007/978-1-4939-8609-5_18

Bioelectronic nose using olfactory receptor-embedded nanodiscs

Heehong Yang, Minju Lee, Daesan Kim, Seunghun Hong, Tai Hyun Park

Department of Nutritional Science & Food Management

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Scopus citations

Abstract

Olfactory receptors (ORs) are the largest family of the G protein-coupled receptors (GPCRs), which are significantly involved in many human diseases and 40% of all drug targets. A platform containing stable and high-quality OR would be a powerful tool for the development of a practical biosensor that can be applied to various applications, such as the early diagnosis of diseases, assessment of food quality, and drug and fragrance development. Significant efforts have been made to develop the biosensor using GPCRs; nevertheless, they remain a challenge. This chapter describes an attractive methodology for the development of a stable bioelectronic nose using OR-embedded nanodiscs. The ORs were produced in Escherichia coli (E. coli), purified with column chromatography, reconstituted into nanodiscs and applied to a carbon nanotube-field effect transistor (CNT-FET) with floating electrodes. The nanodisc-based bioelectronic nose exhibits high-performance in terms of sensitivity, selectivity and stability. This strategy can be used as a practical method for the receptor-based sensing approach, which represents significant progress in nano-bio technology toward a practical biosensor.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	239-249
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-4939-8609-5_18
State	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1820
ISSN (Print)	1064-3745

Bibliographical note

Funding Information:
This research was supported by the National Research Foundation funded by the Korean government (MSIT) (NRF-2018R1A2B3004498). S.H. acknowledges the support from the BioNano Health-Guard Research Center funded by the Ministry of Science and ICT(MSIT) of Korea as Global Frontier Project (Grant number H-GUARD_2013M3A6B2078961) and from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 682286).

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2018.

Keywords

Bioelectronic nose
Carbon nanotube
Field-effect transistor
Nanodisc
Olfactory receptor

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-8609-5_18

Cite this

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title = "Bioelectronic nose using olfactory receptor-embedded nanodiscs",

abstract = "Olfactory receptors (ORs) are the largest family of the G protein-coupled receptors (GPCRs), which are significantly involved in many human diseases and 40% of all drug targets. A platform containing stable and high-quality OR would be a powerful tool for the development of a practical biosensor that can be applied to various applications, such as the early diagnosis of diseases, assessment of food quality, and drug and fragrance development. Significant efforts have been made to develop the biosensor using GPCRs; nevertheless, they remain a challenge. This chapter describes an attractive methodology for the development of a stable bioelectronic nose using OR-embedded nanodiscs. The ORs were produced in Escherichia coli (E. coli), purified with column chromatography, reconstituted into nanodiscs and applied to a carbon nanotube-field effect transistor (CNT-FET) with floating electrodes. The nanodisc-based bioelectronic nose exhibits high-performance in terms of sensitivity, selectivity and stability. This strategy can be used as a practical method for the receptor-based sensing approach, which represents significant progress in nano-bio technology toward a practical biosensor.",

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author = "Heehong Yang and Minju Lee and Daesan Kim and Seunghun Hong and Park, {Tai Hyun}",

note = "Funding Information: This research was supported by the National Research Foundation funded by the Korean government (MSIT) (NRF-2018R1A2B3004498). S.H. acknowledges the support from the BioNano Health-Guard Research Center funded by the Ministry of Science and ICT(MSIT) of Korea as Global Frontier Project (Grant number H-GUARD_2013M3A6B2078961) and from the European Research Council (ERC) under the European Union{\textquoteright}s Horizon 2020 research and innovation programme (grant agreement No. 682286). Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2018.",

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N1 - Funding Information: This research was supported by the National Research Foundation funded by the Korean government (MSIT) (NRF-2018R1A2B3004498). S.H. acknowledges the support from the BioNano Health-Guard Research Center funded by the Ministry of Science and ICT(MSIT) of Korea as Global Frontier Project (Grant number H-GUARD_2013M3A6B2078961) and from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 682286). Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2018.

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N2 - Olfactory receptors (ORs) are the largest family of the G protein-coupled receptors (GPCRs), which are significantly involved in many human diseases and 40% of all drug targets. A platform containing stable and high-quality OR would be a powerful tool for the development of a practical biosensor that can be applied to various applications, such as the early diagnosis of diseases, assessment of food quality, and drug and fragrance development. Significant efforts have been made to develop the biosensor using GPCRs; nevertheless, they remain a challenge. This chapter describes an attractive methodology for the development of a stable bioelectronic nose using OR-embedded nanodiscs. The ORs were produced in Escherichia coli (E. coli), purified with column chromatography, reconstituted into nanodiscs and applied to a carbon nanotube-field effect transistor (CNT-FET) with floating electrodes. The nanodisc-based bioelectronic nose exhibits high-performance in terms of sensitivity, selectivity and stability. This strategy can be used as a practical method for the receptor-based sensing approach, which represents significant progress in nano-bio technology toward a practical biosensor.

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Bioelectronic nose using olfactory receptor-embedded nanodiscs

Abstract

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Bibliographical note

Keywords

UN SDGs

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