Biodistribution and genotoxicity of transferrin-conjugated liposomes/DNA complexes in mice

Mi Lee Sang; Jin Seok Kim; Yu Kyoung Oh; Yong Bok Lee; Hongkee Sah

doi:10.1007/bf03219055

Biodistribution and genotoxicity of transferrin-conjugated liposomes/DNA complexes in mice

Mi Lee Sang, Jin Seok Kim, Yu Kyoung Oh, Yong Bok Lee, Hongkee Sah

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Transferrin-conjugated liposomes (T_f-liposomes) were made and formulated with pCMVluc DNA to form a lipoplex. Among the various formulations studied, the T_f-liposome: pCMVluc DNA complex at a ratio of 5:1 (wt/wt) showed the highest transfection efficiency, which was twice that of Lipofectin™ on HeLa cells. The maximum tolerated dose (MTD) of this lipoplex formulation from a single intravenous injection was over 10 mg/kg in healthy ICR mice. The RT-PCR results showed that the highest level of luciferase mRNA was detected in the lungs, followed by the liver, spleen, heart and kidneys, after an intravenous injection into mice. Two weeks after the injection, the levels of luciferase mRNA decreased gradually in the liver, spleen, heart, and kidney, but not in the lungs. The micro-array study showed that the cancer-related genes, including the bcl 6 gene, were highly up-regulated by the treatment with T_f-liposome/ pCMVluc DNA complex on HeLa cells, indicating that there were possible interactions between the host chromosomal DNA and the T_f-liposome Within the cells. The results obtained from this study are expected to be useful for designing a safe and efficient gene delivery system using transferrin-conjugated liposomes.

Original language	English
Pages (from-to)	218-222
Number of pages	5
Journal	Macromolecular Research
Volume	13
Issue number	3
DOIs	https://doi.org/10.1007/bf03219055
State	Published - Jun 2005

Keywords

Biodistribution
Gene delivery
Microarray
MTD

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Biodistribution and genotoxicity of transferrin-conjugated liposomes/DNA complexes in mice",

abstract = "Transferrin-conjugated liposomes (Tf-liposomes) were made and formulated with pCMVluc DNA to form a lipoplex. Among the various formulations studied, the Tf-liposome: pCMVluc DNA complex at a ratio of 5:1 (wt/wt) showed the highest transfection efficiency, which was twice that of Lipofectin{\texttrademark} on HeLa cells. The maximum tolerated dose (MTD) of this lipoplex formulation from a single intravenous injection was over 10 mg/kg in healthy ICR mice. The RT-PCR results showed that the highest level of luciferase mRNA was detected in the lungs, followed by the liver, spleen, heart and kidneys, after an intravenous injection into mice. Two weeks after the injection, the levels of luciferase mRNA decreased gradually in the liver, spleen, heart, and kidney, but not in the lungs. The micro-array study showed that the cancer-related genes, including the bcl 6 gene, were highly up-regulated by the treatment with Tf-liposome/ pCMVluc DNA complex on HeLa cells, indicating that there were possible interactions between the host chromosomal DNA and the Tf-liposome Within the cells. The results obtained from this study are expected to be useful for designing a safe and efficient gene delivery system using transferrin-conjugated liposomes.",

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author = "Sang, {Mi Lee} and Kim, {Jin Seok} and Oh, {Yu Kyoung} and Lee, {Yong Bok} and Hongkee Sah",

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AU - Lee, Yong Bok

AU - Sah, Hongkee

PY - 2005/6

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N2 - Transferrin-conjugated liposomes (Tf-liposomes) were made and formulated with pCMVluc DNA to form a lipoplex. Among the various formulations studied, the Tf-liposome: pCMVluc DNA complex at a ratio of 5:1 (wt/wt) showed the highest transfection efficiency, which was twice that of Lipofectin™ on HeLa cells. The maximum tolerated dose (MTD) of this lipoplex formulation from a single intravenous injection was over 10 mg/kg in healthy ICR mice. The RT-PCR results showed that the highest level of luciferase mRNA was detected in the lungs, followed by the liver, spleen, heart and kidneys, after an intravenous injection into mice. Two weeks after the injection, the levels of luciferase mRNA decreased gradually in the liver, spleen, heart, and kidney, but not in the lungs. The micro-array study showed that the cancer-related genes, including the bcl 6 gene, were highly up-regulated by the treatment with Tf-liposome/ pCMVluc DNA complex on HeLa cells, indicating that there were possible interactions between the host chromosomal DNA and the Tf-liposome Within the cells. The results obtained from this study are expected to be useful for designing a safe and efficient gene delivery system using transferrin-conjugated liposomes.

AB - Transferrin-conjugated liposomes (Tf-liposomes) were made and formulated with pCMVluc DNA to form a lipoplex. Among the various formulations studied, the Tf-liposome: pCMVluc DNA complex at a ratio of 5:1 (wt/wt) showed the highest transfection efficiency, which was twice that of Lipofectin™ on HeLa cells. The maximum tolerated dose (MTD) of this lipoplex formulation from a single intravenous injection was over 10 mg/kg in healthy ICR mice. The RT-PCR results showed that the highest level of luciferase mRNA was detected in the lungs, followed by the liver, spleen, heart and kidneys, after an intravenous injection into mice. Two weeks after the injection, the levels of luciferase mRNA decreased gradually in the liver, spleen, heart, and kidney, but not in the lungs. The micro-array study showed that the cancer-related genes, including the bcl 6 gene, were highly up-regulated by the treatment with Tf-liposome/ pCMVluc DNA complex on HeLa cells, indicating that there were possible interactions between the host chromosomal DNA and the Tf-liposome Within the cells. The results obtained from this study are expected to be useful for designing a safe and efficient gene delivery system using transferrin-conjugated liposomes.

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Biodistribution and genotoxicity of transferrin-conjugated liposomes/DNA complexes in mice

Abstract

Keywords

UN SDGs

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