Biocompatible Properties and Mineralization Potential of Premixed Calcium Silicate-Based Cements and Fast-Set Calcium Silicate-Based Cements on Human Bone Marrow-Derived Mesenchymal Stem Cells

Yemi Kim, Donghee Lee, Minjoo Kye, Yun Jae Ha, Sin Young Kim

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Premixed calcium silicate-based cements (CSCs) and fast-set CSCs were developed for the convenience of retrograde filling during endodontic microsurgery. The aim of this study was to analyze the biocompatible properties and mineralization potential of premixed CSCs, such as Endocem MTA Premixed (EM Premixed) and EndoSequence BC RRM putty (EndoSequence), and fast-set RetroMTA on human bone marrow-derived mesenchymal stem cells (BMSCs) compared to ProRoot MTA. Using CCK-8, a significantly higher proliferation of BMSCs occurred only in the EM Premixed group on days 2 and 4 (p < 0.05). On day 6, the ProRoot MTA group had significantly higher cell proliferation than the control group (p < 0.05). Regardless of the experimental materials, all groups had complete cell migration by day 4. Alizarin Red-S staining and alkaline phosphatase assay demonstrated higher mineralization potential of all CSCs similar to ProRoot MTA (p < 0.05). The EndoSequence group showed more upregulation of SMAD1 and OSX gene expression than the other experimental groups (p < 0.05), and all experimental cements upregulated osteogenic gene expression more than the control group (p < 0.05). Therefore, using premixed CSCs and fast-set CSCs as retrograde filling cements may facilitate satisfactory biological responses and comparable osteogenic potential to ProRoot MTA.

Original languageEnglish
Article number7595
JournalMaterials
Volume15
Issue number21
DOIs
StatePublished - Nov 2022

Bibliographical note

Funding Information:
This research was supported by a National Research Foundation of Korea (NRF) grant funded by the Ministry of Education (NRF-2021R1I1A2041534) and Research Fund of Seoul St. Mary’s Hospital, The Catholic University of Korea.

Publisher Copyright:
© 2022 by the authors.

Keywords

  • biocompatibility
  • calcium silicate-based cement
  • human bone marrow-derived mesenchymal stem cell
  • mineralization potential

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