Biocompatibility and bioactivity of set direct pulp capping materials on human dental pulp stem cells

Yemi Kim; Donghee Lee; Dani Song; Hye Min Kim; Sin Young Kim

doi:10.3390/MA13183925

Biocompatibility and bioactivity of set direct pulp capping materials on human dental pulp stem cells

Yemi Kim, Donghee Lee, Dani Song, Hye Min Kim, Sin Young Kim

Department of Dentistry

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

In this study, we assessed the biocompatibility and bioactivity of various pulp capping materials-ProRoot MTA (Dentsply Tulsa Dental Specialties), Biodentine (Septodont), TheraCal LC (Bisco), and Dycal (Dentsply Caulk)-on human dental pulp stem cells (hDPSCs). Experimental disks (diameter, 7 mm; height, 4 mm) were stored in a humified incubator at 37 °C for 48 h. Then, the pulp capping materials were tested for cytotoxic effects by methyl-thiazoldiphenyl-tetrazolium and scratch wound healing assays, and for mineralization potential by Alizarin red S (ARS) staining assay and alkaline phosphatase enzyme (ALP) activity. Cell viability and cell migration did not significantly differ between ProRoot MTA, Biodentine, and control (p > 0.05). TheraCal LC exhibited slower cell migration on days 2-4 compared to control (p < 0.05), and Dycal showed no cell migration. ALP activity was highest with Biodentine on days 10 and 14, and was lowered with TheraCal LC and Dycal (p < 0.05). In the ARS assay, hDPSCs grown in ProRoot MTA and TheraCal LC eluates showed significantly increased mineralized nodule formation on day 21 compared to Biodentine, Dycal, and control (p < 0.05). These findings indicate that ProRoot MTA, Biodentine, and TheraCal LC exhibit better biocompatibility and bioactivity than Dycal.

Original language	English
Article number	3925
Journal	Materials
Volume	13
Issue number	18
DOIs	https://doi.org/10.3390/MA13183925
State	Published - Sep 2020

Bibliographical note

Funding Information:
Funding: This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (Ministry of Science, ICT and Future Planning) (no. 2017R1C1B5017098 and 2019R1F1A1058955).

Publisher Copyright:
© 2020 by the authors.

Keywords

ALP activity
ARS assay
Cell migration
Cell viability
Pulp capping materials

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10.3390/MA13183925

Cite this

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title = "Biocompatibility and bioactivity of set direct pulp capping materials on human dental pulp stem cells",

abstract = "In this study, we assessed the biocompatibility and bioactivity of various pulp capping materials-ProRoot MTA (Dentsply Tulsa Dental Specialties), Biodentine (Septodont), TheraCal LC (Bisco), and Dycal (Dentsply Caulk)-on human dental pulp stem cells (hDPSCs). Experimental disks (diameter, 7 mm; height, 4 mm) were stored in a humified incubator at 37 °C for 48 h. Then, the pulp capping materials were tested for cytotoxic effects by methyl-thiazoldiphenyl-tetrazolium and scratch wound healing assays, and for mineralization potential by Alizarin red S (ARS) staining assay and alkaline phosphatase enzyme (ALP) activity. Cell viability and cell migration did not significantly differ between ProRoot MTA, Biodentine, and control (p > 0.05). TheraCal LC exhibited slower cell migration on days 2-4 compared to control (p < 0.05), and Dycal showed no cell migration. ALP activity was highest with Biodentine on days 10 and 14, and was lowered with TheraCal LC and Dycal (p < 0.05). In the ARS assay, hDPSCs grown in ProRoot MTA and TheraCal LC eluates showed significantly increased mineralized nodule formation on day 21 compared to Biodentine, Dycal, and control (p < 0.05). These findings indicate that ProRoot MTA, Biodentine, and TheraCal LC exhibit better biocompatibility and bioactivity than Dycal.",

keywords = "ALP activity, ARS assay, Cell migration, Cell viability, Pulp capping materials",

author = "Yemi Kim and Donghee Lee and Dani Song and Kim, {Hye Min} and Kim, {Sin Young}",

note = "Funding Information: Funding: This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (Ministry of Science, ICT and Future Planning) (no. 2017R1C1B5017098 and 2019R1F1A1058955). Publisher Copyright: {\textcopyright} 2020 by the authors.",

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AU - Kim, Yemi

AU - Lee, Donghee

AU - Song, Dani

AU - Kim, Hye Min

AU - Kim, Sin Young

N1 - Funding Information: Funding: This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (Ministry of Science, ICT and Future Planning) (no. 2017R1C1B5017098 and 2019R1F1A1058955). Publisher Copyright: © 2020 by the authors.

PY - 2020/9

Y1 - 2020/9

N2 - In this study, we assessed the biocompatibility and bioactivity of various pulp capping materials-ProRoot MTA (Dentsply Tulsa Dental Specialties), Biodentine (Septodont), TheraCal LC (Bisco), and Dycal (Dentsply Caulk)-on human dental pulp stem cells (hDPSCs). Experimental disks (diameter, 7 mm; height, 4 mm) were stored in a humified incubator at 37 °C for 48 h. Then, the pulp capping materials were tested for cytotoxic effects by methyl-thiazoldiphenyl-tetrazolium and scratch wound healing assays, and for mineralization potential by Alizarin red S (ARS) staining assay and alkaline phosphatase enzyme (ALP) activity. Cell viability and cell migration did not significantly differ between ProRoot MTA, Biodentine, and control (p > 0.05). TheraCal LC exhibited slower cell migration on days 2-4 compared to control (p < 0.05), and Dycal showed no cell migration. ALP activity was highest with Biodentine on days 10 and 14, and was lowered with TheraCal LC and Dycal (p < 0.05). In the ARS assay, hDPSCs grown in ProRoot MTA and TheraCal LC eluates showed significantly increased mineralized nodule formation on day 21 compared to Biodentine, Dycal, and control (p < 0.05). These findings indicate that ProRoot MTA, Biodentine, and TheraCal LC exhibit better biocompatibility and bioactivity than Dycal.

AB - In this study, we assessed the biocompatibility and bioactivity of various pulp capping materials-ProRoot MTA (Dentsply Tulsa Dental Specialties), Biodentine (Septodont), TheraCal LC (Bisco), and Dycal (Dentsply Caulk)-on human dental pulp stem cells (hDPSCs). Experimental disks (diameter, 7 mm; height, 4 mm) were stored in a humified incubator at 37 °C for 48 h. Then, the pulp capping materials were tested for cytotoxic effects by methyl-thiazoldiphenyl-tetrazolium and scratch wound healing assays, and for mineralization potential by Alizarin red S (ARS) staining assay and alkaline phosphatase enzyme (ALP) activity. Cell viability and cell migration did not significantly differ between ProRoot MTA, Biodentine, and control (p > 0.05). TheraCal LC exhibited slower cell migration on days 2-4 compared to control (p < 0.05), and Dycal showed no cell migration. ALP activity was highest with Biodentine on days 10 and 14, and was lowered with TheraCal LC and Dycal (p < 0.05). In the ARS assay, hDPSCs grown in ProRoot MTA and TheraCal LC eluates showed significantly increased mineralized nodule formation on day 21 compared to Biodentine, Dycal, and control (p < 0.05). These findings indicate that ProRoot MTA, Biodentine, and TheraCal LC exhibit better biocompatibility and bioactivity than Dycal.

KW - ALP activity

KW - ARS assay

KW - Cell migration

KW - Cell viability

KW - Pulp capping materials

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U2 - 10.3390/MA13183925

DO - 10.3390/MA13183925

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SN - 1996-1944

VL - 13

JO - Materials

JF - Materials

IS - 18

M1 - 3925

ER -

Biocompatibility and bioactivity of set direct pulp capping materials on human dental pulp stem cells

Abstract

Bibliographical note

Keywords

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