Abstract
Silkworm hemolymph presents various beneficial effects on the cultivation of insect cell-baculovirus system. It can be used as a substitute for fetal bovine serum which is a medium supplement. It increases the recombinant protein expression rate and host cell longevity after baculoviral infection. The increase in the host cell longevity is due to its apoptosis-inhibition activity. There are several components in the silkworm hemolymph which have apoptosis-inhibition activity. The component with highest activity is a non-glycosylated monomeric protein with the molecular weight of ca. 28,000 Da.
| Original language | English |
|---|---|
| Pages (from-to) | 153-162 |
| Number of pages | 10 |
| Journal | ACS Symposium Series |
| Volume | 830 |
| DOIs | |
| State | Published - 2002 |