TY - JOUR
T1 - Beneficial effect of silkworm hemolymph on a CHO cell system
T2 - Inhibition of apoptosis and increase of EPO production
AU - Choi, Shin Sik
AU - Rhee, Won Jong
AU - Park, Tai Hyun
PY - 2005/9/30
Y1 - 2005/9/30
N2 - To produce erythropoietin (EPO), Chinese hamster ovary (CHO) cells were first cultured in a medium containing FBS (growth medium) and then in a serum-free medium containing sodium butyrate (production medium). Sodium butyrate increases recombinant protein production, but also induces apoptosis, which reduces cell viability and productivity. In a previous study, we found that silkworm hemolymph (SH), an insect serum, inhibits the apoptosis of insect and mammalian cells. To overcome sodium butyrate-induced apoptosis, we added SH to growth medium. This pretreatment with SH inhibited the sodium butyrate-induced apoptosis of CHO cells and consequently increased their longevity and their ability to produce EPO. As a result, the volumetric productivity of EPO was increased five-fold. SH was found to inhibit cytochrome c release from mitochondria into the cytosol, and prevented the activation of caspase-3 and other subsequent caspase reactions.
AB - To produce erythropoietin (EPO), Chinese hamster ovary (CHO) cells were first cultured in a medium containing FBS (growth medium) and then in a serum-free medium containing sodium butyrate (production medium). Sodium butyrate increases recombinant protein production, but also induces apoptosis, which reduces cell viability and productivity. In a previous study, we found that silkworm hemolymph (SH), an insect serum, inhibits the apoptosis of insect and mammalian cells. To overcome sodium butyrate-induced apoptosis, we added SH to growth medium. This pretreatment with SH inhibited the sodium butyrate-induced apoptosis of CHO cells and consequently increased their longevity and their ability to produce EPO. As a result, the volumetric productivity of EPO was increased five-fold. SH was found to inhibit cytochrome c release from mitochondria into the cytosol, and prevented the activation of caspase-3 and other subsequent caspase reactions.
KW - Apoptosis
KW - Chinese hamster ovary (CHO)
KW - Erythropoietin (EPO)
KW - Silkworm hemolymph (SH)
KW - Sodium butyrate
UR - http://www.scopus.com/inward/record.url?scp=25144503429&partnerID=8YFLogxK
U2 - 10.1002/bit.20550
DO - 10.1002/bit.20550
M3 - Article
C2 - 15948145
AN - SCOPUS:25144503429
SN - 0006-3592
VL - 91
SP - 793
EP - 800
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
IS - 7
ER -