TY - JOUR
T1 - Bead-immobilized molecular beacons for high throughput snp genotyping via a microfluidic system
AU - Sochol, R. D.
AU - Mahajerin, A.
AU - Casavant, B. P.
AU - Singh, P.
AU - Dueck, M.
AU - Lee, L. P.
AU - Lin, L.
PY - 2009
Y1 - 2009
N2 - This paper reports the first demonstration of single nucleotide polymorphism (SNP) genotyping via molecular beacon probes immobilized on polystyrene microbead substrates within a dynamic microfluidic system. Additionally, we present an optimized bead immobilization technique, micropost array trapping (μPAT), for high-density and high-throughput arraying of beads. Quantitative detection was achieved at room temperature for three label-free DNA oligonucleotide sequences based on the genome of the Hepatitis C Virus (HCV) in humans. SNP detection technology is integral for the realization of personalized medicine and genetic disease identification, and this system offers a promising technique for fast, sensitive, and cost-effective SNP genotyping.
AB - This paper reports the first demonstration of single nucleotide polymorphism (SNP) genotyping via molecular beacon probes immobilized on polystyrene microbead substrates within a dynamic microfluidic system. Additionally, we present an optimized bead immobilization technique, micropost array trapping (μPAT), for high-density and high-throughput arraying of beads. Quantitative detection was achieved at room temperature for three label-free DNA oligonucleotide sequences based on the genome of the Hepatitis C Virus (HCV) in humans. SNP detection technology is integral for the realization of personalized medicine and genetic disease identification, and this system offers a promising technique for fast, sensitive, and cost-effective SNP genotyping.
UR - https://www.scopus.com/pages/publications/65949083800
U2 - 10.1109/MEMSYS.2009.4805379
DO - 10.1109/MEMSYS.2009.4805379
M3 - Conference article
AN - SCOPUS:65949083800
SN - 1084-6999
SP - 304
EP - 307
JO - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
JF - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
M1 - 4805379
T2 - 22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009
Y2 - 25 January 2009 through 29 January 2009
ER -
