Bead-immobilized molecular beacons for high throughput snp genotyping via a microfluidic system

R. D. Sochol; A. Mahajerin; B. P. Casavant; P. Singh; M. Dueck; L. P. Lee; L. Lin

doi:10.1109/MEMSYS.2009.4805379

Bead-immobilized molecular beacons for high throughput snp genotyping via a microfluidic system

R. D. Sochol
, A. Mahajerin
, B. P. Casavant
, P. Singh
, M. Dueck
, L. P. Lee
, L. Lin

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Conference article › peer-review

6 Scopus citations

Abstract

This paper reports the first demonstration of single nucleotide polymorphism (SNP) genotyping via molecular beacon probes immobilized on polystyrene microbead substrates within a dynamic microfluidic system. Additionally, we present an optimized bead immobilization technique, micropost array trapping (μPAT), for high-density and high-throughput arraying of beads. Quantitative detection was achieved at room temperature for three label-free DNA oligonucleotide sequences based on the genome of the Hepatitis C Virus (HCV) in humans. SNP detection technology is integral for the realization of personalized medicine and genetic disease identification, and this system offers a promising technique for fast, sensitive, and cost-effective SNP genotyping.

Original language	English
Article number	4805379
Pages (from-to)	304-307
Number of pages	4
Journal	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
DOIs	https://doi.org/10.1109/MEMSYS.2009.4805379
State	Published - 2009
Event	22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009 - Sorrento, Italy Duration: 25 Jan 2009 → 29 Jan 2009

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title = "Bead-immobilized molecular beacons for high throughput snp genotyping via a microfluidic system",

abstract = "This paper reports the first demonstration of single nucleotide polymorphism (SNP) genotyping via molecular beacon probes immobilized on polystyrene microbead substrates within a dynamic microfluidic system. Additionally, we present an optimized bead immobilization technique, micropost array trapping (μPAT), for high-density and high-throughput arraying of beads. Quantitative detection was achieved at room temperature for three label-free DNA oligonucleotide sequences based on the genome of the Hepatitis C Virus (HCV) in humans. SNP detection technology is integral for the realization of personalized medicine and genetic disease identification, and this system offers a promising technique for fast, sensitive, and cost-effective SNP genotyping.",

author = "Sochol, \{R. D.\} and A. Mahajerin and Casavant, \{B. P.\} and P. Singh and M. Dueck and Lee, \{L. P.\} and L. Lin",

year = "2009",

doi = "10.1109/MEMSYS.2009.4805379",

language = "English",

pages = "304--307",

journal = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

issn = "1084-6999",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

note = "22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009 ; Conference date: 25-01-2009 Through 29-01-2009",

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TY - JOUR

T1 - Bead-immobilized molecular beacons for high throughput snp genotyping via a microfluidic system

AU - Sochol, R. D.

AU - Mahajerin, A.

AU - Casavant, B. P.

AU - Singh, P.

AU - Dueck, M.

AU - Lee, L. P.

AU - Lin, L.

PY - 2009

Y1 - 2009

N2 - This paper reports the first demonstration of single nucleotide polymorphism (SNP) genotyping via molecular beacon probes immobilized on polystyrene microbead substrates within a dynamic microfluidic system. Additionally, we present an optimized bead immobilization technique, micropost array trapping (μPAT), for high-density and high-throughput arraying of beads. Quantitative detection was achieved at room temperature for three label-free DNA oligonucleotide sequences based on the genome of the Hepatitis C Virus (HCV) in humans. SNP detection technology is integral for the realization of personalized medicine and genetic disease identification, and this system offers a promising technique for fast, sensitive, and cost-effective SNP genotyping.

AB - This paper reports the first demonstration of single nucleotide polymorphism (SNP) genotyping via molecular beacon probes immobilized on polystyrene microbead substrates within a dynamic microfluidic system. Additionally, we present an optimized bead immobilization technique, micropost array trapping (μPAT), for high-density and high-throughput arraying of beads. Quantitative detection was achieved at room temperature for three label-free DNA oligonucleotide sequences based on the genome of the Hepatitis C Virus (HCV) in humans. SNP detection technology is integral for the realization of personalized medicine and genetic disease identification, and this system offers a promising technique for fast, sensitive, and cost-effective SNP genotyping.

UR - https://www.scopus.com/pages/publications/65949083800

U2 - 10.1109/MEMSYS.2009.4805379

DO - 10.1109/MEMSYS.2009.4805379

M3 - Conference article

AN - SCOPUS:65949083800

SN - 1084-6999

SP - 304

EP - 307

JO - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

JF - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

M1 - 4805379

T2 - 22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009

Y2 - 25 January 2009 through 29 January 2009

ER -

Bead-immobilized molecular beacons for high throughput snp genotyping via a microfluidic system

Abstract

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