This paper reports the first demonstration of single nucleotide polymorphism (SNP) genotyping via molecular beacon probes immobilized on polystyrene microbead substrates within a dynamic microfluidic system. Additionally, we present an optimized bead immobilization technique, micropost array trapping (μPAT), for high-density and high-throughput arraying of beads. Quantitative detection was achieved at room temperature for three label-free DNA oligonucleotide sequences based on the genome of the Hepatitis C Virus (HCV) in humans. SNP detection technology is integral for the realization of personalized medicine and genetic disease identification, and this system offers a promising technique for fast, sensitive, and cost-effective SNP genotyping.
|Number of pages||4|
|Journal||Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)|
|State||Published - 2009|
|Event||22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009 - Sorrento, Italy|
Duration: 25 Jan 2009 → 29 Jan 2009