Bead-immobilized molecular beacons for high throughput snp genotyping via a microfluidic system

R. D. Sochol, A. Mahajerin, B. P. Casavant, P. Singh, M. Dueck, L. P. Lee, L. Lin

Research output: Contribution to journalConference articlepeer-review

6 Scopus citations

Abstract

This paper reports the first demonstration of single nucleotide polymorphism (SNP) genotyping via molecular beacon probes immobilized on polystyrene microbead substrates within a dynamic microfluidic system. Additionally, we present an optimized bead immobilization technique, micropost array trapping (μPAT), for high-density and high-throughput arraying of beads. Quantitative detection was achieved at room temperature for three label-free DNA oligonucleotide sequences based on the genome of the Hepatitis C Virus (HCV) in humans. SNP detection technology is integral for the realization of personalized medicine and genetic disease identification, and this system offers a promising technique for fast, sensitive, and cost-effective SNP genotyping.

Original languageEnglish
Article number4805379
Pages (from-to)304-307
Number of pages4
JournalProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
DOIs
StatePublished - 2009
Event22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009 - Sorrento, Italy
Duration: 25 Jan 200929 Jan 2009

Fingerprint

Dive into the research topics of 'Bead-immobilized molecular beacons for high throughput snp genotyping via a microfluidic system'. Together they form a unique fingerprint.

Cite this