@article{82760829fc444461b08f1f049a5518bf,
title = "Bacterial Outer Membrane Vesicles as Nano-Scale Bioreactors: A Fatty Acid Conversion Case Study",
abstract = "Bacterial outer membrane vesicles (OMVs) are small unilamellar proteoliposomes, involved in various functions including cell-to-cell signalling and protein excretion. We have engineered the OMVs of Escherichia coli to nano-scaled bioreactors for the biotransformation of fatty acids by targeting a fatty acid double bond hydratase of Stentrophomonas maltophilia (SmOhyA) and/or a photoactivated fatty acid decarboxylase from Chlorella variabilis NC64 A (CvFAP) into OMVs. Engineered OMVs containing both SmOhyA and CvFAP were able to catalyse the transformation of oleic acid ((Z)-octadec-9-enoic acid) into 9-hydroxyheptadecane via (R)-10-hydroxyoctadecanoic acid. The specific biotransformation rates of oleic acid reached 8.0×10−12 μmol/min per OMV.",
keywords = "biocatalysis, decarboxylase, hydratase, nano-scale bioreactor, outer membrane vesicles",
author = "Song, {Ji Won} and Yoonjin Baeg and Jeong, {Ha Yeon} and Jinwon Lee and Oh, {Deok Kun} and Frank Hollmann and Park, {Jin Byung}",
note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (No. 2020R1A2B5B03002376) and by C1 Gas Refinery Research Center of the NRF funded by Ministry of Science and ICT, Korea (Grant Number: 2018M3D3A1A01055735). J.‐W. Song was partially supported by RP‐Grant of Ewha Womans University. Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (No. 2020R1A2B5B03002376) and by C1 Gas Refinery Research Center of the NRF funded by Ministry of Science and ICT, Korea (Grant Number: 2018M3D3A1A01055735). J.-W. Song was partially supported by RP-Grant of Ewha Womans University. Publisher Copyright: {\textcopyright} 2021 The Authors. ChemCatChem published by Wiley-VCH GmbH",
year = "2021",
month = oct,
day = "7",
doi = "10.1002/cctc.202100778",
language = "English",
volume = "13",
pages = "4080--4086",
journal = "ChemCatChem",
issn = "1867-3880",
publisher = "Wiley - VCH Verlag GmbH & CO. KGaA",
number = "19",
}