Bacterial Outer Membrane Vesicles as Nano-Scale Bioreactors: A Fatty Acid Conversion Case Study

Ji Won Song; Yoonjin Baeg; Ha Yeon Jeong; Jinwon Lee; Deok Kun Oh; Frank Hollmann; Jin Byung Park

doi:10.1002/cctc.202100778

Bacterial Outer Membrane Vesicles as Nano-Scale Bioreactors: A Fatty Acid Conversion Case Study

Ji Won Song, Yoonjin Baeg, Ha Yeon Jeong, Jinwon Lee, Deok Kun Oh, Frank Hollmann, Jin Byung Park

Department of Food Science & Biotechnology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Bacterial outer membrane vesicles (OMVs) are small unilamellar proteoliposomes, involved in various functions including cell-to-cell signalling and protein excretion. We have engineered the OMVs of Escherichia coli to nano-scaled bioreactors for the biotransformation of fatty acids by targeting a fatty acid double bond hydratase of Stentrophomonas maltophilia (SmOhyA) and/or a photoactivated fatty acid decarboxylase from Chlorella variabilis NC64 A (CvFAP) into OMVs. Engineered OMVs containing both SmOhyA and CvFAP were able to catalyse the transformation of oleic acid ((Z)-octadec-9-enoic acid) into 9-hydroxyheptadecane via (R)-10-hydroxyoctadecanoic acid. The specific biotransformation rates of oleic acid reached 8.0×10⁻¹² μmol/min per OMV.

Original language	English
Pages (from-to)	4080-4086
Number of pages	7
Journal	ChemCatChem
Volume	13
Issue number	19
DOIs	https://doi.org/10.1002/cctc.202100778
State	Published - 7 Oct 2021

Bibliographical note

Publisher Copyright:
© 2021 The Authors. ChemCatChem published by Wiley-VCH GmbH

Keywords

biocatalysis
decarboxylase
hydratase
nano-scale bioreactor
outer membrane vesicles

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title = "Bacterial Outer Membrane Vesicles as Nano-Scale Bioreactors: A Fatty Acid Conversion Case Study",

abstract = "Bacterial outer membrane vesicles (OMVs) are small unilamellar proteoliposomes, involved in various functions including cell-to-cell signalling and protein excretion. We have engineered the OMVs of Escherichia coli to nano-scaled bioreactors for the biotransformation of fatty acids by targeting a fatty acid double bond hydratase of Stentrophomonas maltophilia (SmOhyA) and/or a photoactivated fatty acid decarboxylase from Chlorella variabilis NC64 A (CvFAP) into OMVs. Engineered OMVs containing both SmOhyA and CvFAP were able to catalyse the transformation of oleic acid ((Z)-octadec-9-enoic acid) into 9-hydroxyheptadecane via (R)-10-hydroxyoctadecanoic acid. The specific biotransformation rates of oleic acid reached 8.0×10−12 μmol/min per OMV.",

keywords = "biocatalysis, decarboxylase, hydratase, nano-scale bioreactor, outer membrane vesicles",

author = "Song, {Ji Won} and Yoonjin Baeg and Jeong, {Ha Yeon} and Jinwon Lee and Oh, {Deok Kun} and Frank Hollmann and Park, {Jin Byung}",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors. ChemCatChem published by Wiley-VCH GmbH",

year = "2021",

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AU - Song, Ji Won

AU - Baeg, Yoonjin

AU - Jeong, Ha Yeon

AU - Lee, Jinwon

AU - Oh, Deok Kun

AU - Hollmann, Frank

AU - Park, Jin Byung

PY - 2021/10/7

Y1 - 2021/10/7

N2 - Bacterial outer membrane vesicles (OMVs) are small unilamellar proteoliposomes, involved in various functions including cell-to-cell signalling and protein excretion. We have engineered the OMVs of Escherichia coli to nano-scaled bioreactors for the biotransformation of fatty acids by targeting a fatty acid double bond hydratase of Stentrophomonas maltophilia (SmOhyA) and/or a photoactivated fatty acid decarboxylase from Chlorella variabilis NC64 A (CvFAP) into OMVs. Engineered OMVs containing both SmOhyA and CvFAP were able to catalyse the transformation of oleic acid ((Z)-octadec-9-enoic acid) into 9-hydroxyheptadecane via (R)-10-hydroxyoctadecanoic acid. The specific biotransformation rates of oleic acid reached 8.0×10−12 μmol/min per OMV.

AB - Bacterial outer membrane vesicles (OMVs) are small unilamellar proteoliposomes, involved in various functions including cell-to-cell signalling and protein excretion. We have engineered the OMVs of Escherichia coli to nano-scaled bioreactors for the biotransformation of fatty acids by targeting a fatty acid double bond hydratase of Stentrophomonas maltophilia (SmOhyA) and/or a photoactivated fatty acid decarboxylase from Chlorella variabilis NC64 A (CvFAP) into OMVs. Engineered OMVs containing both SmOhyA and CvFAP were able to catalyse the transformation of oleic acid ((Z)-octadec-9-enoic acid) into 9-hydroxyheptadecane via (R)-10-hydroxyoctadecanoic acid. The specific biotransformation rates of oleic acid reached 8.0×10−12 μmol/min per OMV.

KW - biocatalysis

KW - decarboxylase

KW - hydratase

KW - nano-scale bioreactor

KW - outer membrane vesicles

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DO - 10.1002/cctc.202100778

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JF - ChemCatChem

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ER -

Bacterial Outer Membrane Vesicles as Nano-Scale Bioreactors: A Fatty Acid Conversion Case Study

Abstract

Bibliographical note

Keywords

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