Bacterial Nucleoside Catabolism Controls Quorum Sensing and Commensal-to-Pathogen Transition in the Drosophila Gut

Eun Kyoung Kim, Kyung Ah Lee, Do Young Hyeon, Minsoo Kyung, Kyu Yeon Jun, Seung Hee Seo, Daehee Hwang, Youngjoo Kwon, Won Jae Lee

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Although the gut microbiome is generally symbiotic or commensal, some microbiome members become pathogenic under certain circumstances. However, the factors driving this pathogenic switch are largely unknown. Pathogenic bacteria can generate uracil that triggers host dual oxidase (DUOX) to produce antimicrobial reactive oxygen species (ROS). We show that pathogens generate uracil and ribose upon nucleoside catabolism of gut luminal uridine, which triggers not only host defenses but also inter-bacterial communication and pathogenesis in Drosophila. Uridine-derived uracil triggers DUOX-dependent ROS generation, whereas ribose induces bacterial quorum sensing (QS) and virulence gene expression. Genes implicated in nucleotide metabolism are found in pathogens but not commensal bacteria, and their genetic ablation blocks QS and the commensal-to-pathogen transition in vivo. Furthermore, commensal bacteria lack functional nucleoside catabolism, which is required to achieve gut-microbe symbiosis, but can become pathogenic by enabling nucleotide catabolism. These findings reveal molecular mechanisms governing the commensal-to-pathogen transition in different contexts of host-microbe interactions.

Original languageEnglish
Pages (from-to)345-357.e6
JournalCell Host and Microbe
Volume27
Issue number3
DOIs
StatePublished - 11 Mar 2020

Keywords

  • Drosophila
  • dual oxidase
  • gut
  • nucleoside hydrolase
  • quorum sensing
  • reactive oxygen species
  • uracil
  • uridine

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