Abstract
Bacterial diversity dynamics were investigated in the soil samples in different distances and depths from/at a long-term petroleumcontaminated site. Microbial activity in the soil samples showed ATP values closely correlated with organic matter content (OC) and total petroleum hydrocarbon (TPH). Bacterial community diversity (H) and evenness (J) using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) and PCR-T-RFLP (terminal restriction fragment length polymorphism) results showed positive correlation with concentration of TPH or OC, but tmoA (toluene monooxygenase gene)-based bacterial H and J using a PCR-T-RFLP result did not. No significant difference of H and J values in the bacterial and the tmoA communities was observed. The bacterial community structure characterized by PCR-DGGE and PCR-T-RFLP techniques showed similarity according to soil sampling distance rather than soil sampling depth. Canonical correspondence analysis demonstrated that OC including TPH had the most significant effect on the bacterial community diversity at the long-term petroleum-contaminated site.
Original language | English |
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Pages (from-to) | 281-290 |
Number of pages | 10 |
Journal | Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering |
Volume | 46 |
Issue number | 3 |
DOIs | |
State | Published - Feb 2011 |
Keywords
- Bacterial community
- Contaminated soil
- Correlation analysis
- Petroleum hydrocarbon