TY - JOUR
T1 - Azulene-Derived Fluorescent Probe for Bioimaging
T2 - Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy
AU - Murfin, Lloyd C.
AU - Weber, Maria
AU - Park, Sang Jun
AU - Kim, Won Tae
AU - Lopez-Alled, Carlos M.
AU - McMullin, Claire L.
AU - Pradaux-Caggiano, Fabienne
AU - Lyall, Catherine L.
AU - Kociok-Köhn, Gabriele
AU - Wenk, Jannis
AU - Bull, Steven D.
AU - Yoon, Juyoung
AU - Kim, Hwan Myung
AU - James, Tony D.
AU - Lewis, Simon E.
N1 - Publisher Copyright:
© 2019 American Chemical Society.
PY - 2019/12/11
Y1 - 2019/12/11
N2 - Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability.
AB - Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability.
UR - https://www.scopus.com/pages/publications/85076384021
U2 - 10.1021/jacs.9b09813
DO - 10.1021/jacs.9b09813
M3 - Article
C2 - 31773957
AN - SCOPUS:85076384021
SN - 0002-7863
VL - 141
SP - 19389
EP - 19396
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 49
ER -