Auxin-induced reactive oxygen species production requires the activation of phosphatidylinositol 3-kinase

Jung Hee Joo; Ho Jung Yoo; Inhwan Hwang; June Seung Lee; Kyoung Hee Nam; Yun Soo Bae

doi:10.1016/j.febslet.2005.01.018

Auxin-induced reactive oxygen species production requires the activation of phosphatidylinositol 3-kinase

Jung Hee Joo, Ho Jung Yoo, Inhwan Hwang, June Seung Lee, Kyoung Hee Nam, Yun Soo Bae

Department of Life Science

Research output: Contribution to journal › Article › peer-review

124 Scopus citations

Abstract

We recently reported that production of reactive oxygen species (ROS) is essential for auxin-induced gravitropic signaling. Here, we investigated the role of phosphatidylinositol 3-kinase and its product, PtdIns(3)P, in auxin-mediated ROS production and the root gravitropic response. Pretreatment with LY294002, an inhibitor of PtdIns 3-kinase activity, blocked auxin-mediated ROS generation, and reduced the sensitivity of root tissue to gravistimulation. The amount of PtdIns(3)P increased in response to auxin, and this effect was abolished by pretreatment with LY294002. In addition, sequestration of PtdIns(3)P by transient expression of the endosome binding domain in protoplasts abrogated IAA-induced ROS accumulation. These results indicate that activation of PtdIns 3-kinase and its product PtdIns(3)P are required for auxin-induced production of ROS and root gravitropism.

Original language	English
Pages (from-to)	1243-1248
Number of pages	6
Journal	FEBS Letters
Volume	579
Issue number	5
DOIs	https://doi.org/10.1016/j.febslet.2005.01.018
State	Published - 14 Feb 2005

Bibliographical note

Funding Information:
We thank Drs. Bin Goo Kang, Jong Sub Lee, Yang Do Choi, Chris Wolverton and Michael Evans for helpful discussions and encouragement during the course of this research. We also gratefully acknowledge Dr. Nina Fedoroff for critical reading of the manuscript. This work was supported by the Korea Science and Engineering Foundation (KOSEF) through the Center for Cell Signaling Research at Ewha Womans University and Crop Functional Genomics Center (Grant CG133 to Y.S.B.), and by a grant from KOSEF to the Environmental Biotechnology Research Center (Grant R15-2003-012-01003-0 to K.H.N). K.H.N was a recipient of a grant from the Brain Pool (031-1-17) of the Korean Federation of Science and Technology Societies, and J.H.J. was the recipient of a BK21 postdoctoral fellowship.

Keywords

Arabidopsis thaliana
Gravitropism
Maize
Phosphatidylinositol 3-kinase
Phosphatidylinositol(3)phosphate
Reactive oxygen species

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10.1016/j.febslet.2005.01.018

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title = "Auxin-induced reactive oxygen species production requires the activation of phosphatidylinositol 3-kinase",

abstract = "We recently reported that production of reactive oxygen species (ROS) is essential for auxin-induced gravitropic signaling. Here, we investigated the role of phosphatidylinositol 3-kinase and its product, PtdIns(3)P, in auxin-mediated ROS production and the root gravitropic response. Pretreatment with LY294002, an inhibitor of PtdIns 3-kinase activity, blocked auxin-mediated ROS generation, and reduced the sensitivity of root tissue to gravistimulation. The amount of PtdIns(3)P increased in response to auxin, and this effect was abolished by pretreatment with LY294002. In addition, sequestration of PtdIns(3)P by transient expression of the endosome binding domain in protoplasts abrogated IAA-induced ROS accumulation. These results indicate that activation of PtdIns 3-kinase and its product PtdIns(3)P are required for auxin-induced production of ROS and root gravitropism.",

keywords = "Arabidopsis thaliana, Gravitropism, Maize, Phosphatidylinositol 3-kinase, Phosphatidylinositol(3)phosphate, Reactive oxygen species",

author = "Joo, {Jung Hee} and Yoo, {Ho Jung} and Inhwan Hwang and Lee, {June Seung} and Nam, {Kyoung Hee} and Bae, {Yun Soo}",

note = "Funding Information: We thank Drs. Bin Goo Kang, Jong Sub Lee, Yang Do Choi, Chris Wolverton and Michael Evans for helpful discussions and encouragement during the course of this research. We also gratefully acknowledge Dr. Nina Fedoroff for critical reading of the manuscript. This work was supported by the Korea Science and Engineering Foundation (KOSEF) through the Center for Cell Signaling Research at Ewha Womans University and Crop Functional Genomics Center (Grant CG133 to Y.S.B.), and by a grant from KOSEF to the Environmental Biotechnology Research Center (Grant R15-2003-012-01003-0 to K.H.N). K.H.N was a recipient of a grant from the Brain Pool (031-1-17) of the Korean Federation of Science and Technology Societies, and J.H.J. was the recipient of a BK21 postdoctoral fellowship. ",

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AU - Nam, Kyoung Hee

AU - Bae, Yun Soo

N1 - Funding Information: We thank Drs. Bin Goo Kang, Jong Sub Lee, Yang Do Choi, Chris Wolverton and Michael Evans for helpful discussions and encouragement during the course of this research. We also gratefully acknowledge Dr. Nina Fedoroff for critical reading of the manuscript. This work was supported by the Korea Science and Engineering Foundation (KOSEF) through the Center for Cell Signaling Research at Ewha Womans University and Crop Functional Genomics Center (Grant CG133 to Y.S.B.), and by a grant from KOSEF to the Environmental Biotechnology Research Center (Grant R15-2003-012-01003-0 to K.H.N). K.H.N was a recipient of a grant from the Brain Pool (031-1-17) of the Korean Federation of Science and Technology Societies, and J.H.J. was the recipient of a BK21 postdoctoral fellowship.

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N2 - We recently reported that production of reactive oxygen species (ROS) is essential for auxin-induced gravitropic signaling. Here, we investigated the role of phosphatidylinositol 3-kinase and its product, PtdIns(3)P, in auxin-mediated ROS production and the root gravitropic response. Pretreatment with LY294002, an inhibitor of PtdIns 3-kinase activity, blocked auxin-mediated ROS generation, and reduced the sensitivity of root tissue to gravistimulation. The amount of PtdIns(3)P increased in response to auxin, and this effect was abolished by pretreatment with LY294002. In addition, sequestration of PtdIns(3)P by transient expression of the endosome binding domain in protoplasts abrogated IAA-induced ROS accumulation. These results indicate that activation of PtdIns 3-kinase and its product PtdIns(3)P are required for auxin-induced production of ROS and root gravitropism.

AB - We recently reported that production of reactive oxygen species (ROS) is essential for auxin-induced gravitropic signaling. Here, we investigated the role of phosphatidylinositol 3-kinase and its product, PtdIns(3)P, in auxin-mediated ROS production and the root gravitropic response. Pretreatment with LY294002, an inhibitor of PtdIns 3-kinase activity, blocked auxin-mediated ROS generation, and reduced the sensitivity of root tissue to gravistimulation. The amount of PtdIns(3)P increased in response to auxin, and this effect was abolished by pretreatment with LY294002. In addition, sequestration of PtdIns(3)P by transient expression of the endosome binding domain in protoplasts abrogated IAA-induced ROS accumulation. These results indicate that activation of PtdIns 3-kinase and its product PtdIns(3)P are required for auxin-induced production of ROS and root gravitropism.

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KW - Gravitropism

KW - Maize

KW - Phosphatidylinositol 3-kinase

KW - Phosphatidylinositol(3)phosphate

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JF - FEBS Letters

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ER -

Auxin-induced reactive oxygen species production requires the activation of phosphatidylinositol 3-kinase

Abstract

Bibliographical note

Keywords

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