Auxin-induced reactive oxygen species production requires the activation of phosphatidylinositol 3-kinase

Jung Hee Joo, Ho Jung Yoo, Inhwan Hwang, June Seung Lee, Kyoung Hee Nam, Yun Soo Bae

Research output: Contribution to journalArticlepeer-review

113 Scopus citations

Abstract

We recently reported that production of reactive oxygen species (ROS) is essential for auxin-induced gravitropic signaling. Here, we investigated the role of phosphatidylinositol 3-kinase and its product, PtdIns(3)P, in auxin-mediated ROS production and the root gravitropic response. Pretreatment with LY294002, an inhibitor of PtdIns 3-kinase activity, blocked auxin-mediated ROS generation, and reduced the sensitivity of root tissue to gravistimulation. The amount of PtdIns(3)P increased in response to auxin, and this effect was abolished by pretreatment with LY294002. In addition, sequestration of PtdIns(3)P by transient expression of the endosome binding domain in protoplasts abrogated IAA-induced ROS accumulation. These results indicate that activation of PtdIns 3-kinase and its product PtdIns(3)P are required for auxin-induced production of ROS and root gravitropism.

Original languageEnglish
Pages (from-to)1243-1248
Number of pages6
JournalFEBS Letters
Volume579
Issue number5
DOIs
StatePublished - 14 Feb 2005

Keywords

  • Arabidopsis thaliana
  • Gravitropism
  • Maize
  • Phosphatidylinositol 3-kinase
  • Phosphatidylinositol(3)phosphate
  • Reactive oxygen species

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