Autoradiography of [3H]glutamate binding during pentobarbital tolerance and withdrawal in the rat

Choon Gon Jang, Seikwan Oh, Hong Zhu, Ing Kang Ho

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


The influence of centrally administered pentobarbital on [3H]glutamate receptor binding in the rat brain was examined. Animals were rendered tolerant by intracerebroventricular (i.c.v.) infusion through osmotic minipumps with pentobarbital (300 μg/10 μl/h, for 6 days), and dependent, by 24 h after withdrawal from pentobarbital. Pentobarbital tolerant rats have significant increases in [3H]glutamate binding in the cortex and hippocampus area. Pentobarbital withdrawal produced increases in glutamate binding in many regions, e.g., the cortex, hippocampus area, thalamus, and cerebellum. These results show that chronic i.c.v, infusion with pentobarbital increases N-methyl-D-aspartate (NMDA) displaceable [3H]glutamate binding, suggesting that an increase in NMDA binding sites may play an important role in the development of tolerance to and withdrawal from pentobarbital.

Original languageEnglish
Pages (from-to)99-102
Number of pages4
JournalBrain Research Bulletin
Issue number1
StatePublished - 1 Jan 1999

Bibliographical note

Funding Information:
The authors thank Dr. T. Ma for his technical assistance and Dr. Susan E. Wellman for her helpful review of the manuscript. This work was supported by a grant NIDA 04480.


  • Autoradiography
  • Glutamate
  • Pentobarbital
  • Tolerance
  • Withdrawal


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