Augmentation of urea-synthetic capacity by inhibition of nitric oxide synthesis in butyrate-induced differentiated human hepatocytes

Jung Hwan Yoon, Hyo Suk Lee, Tae Hun Kim, Gwang Hoon Woo, Chung Yong Kim

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11 Scopus citations

Abstract

We have recently developed an in vitro differentiation model of immortalized non-transformed human hepatocytes using butyrate, and observed the induction of inducible NO synthase (iNOS). In this study, we analyzed the effect of NO on the urea-synthetic capacity of these cells. The inhibition of iNOS during butyrate treatment significantly increased the urea-synthetic capacity as compared to that of butyrate treatment alone, possibly through the further induction of ornithine transcarbamylase expression. Therefore, the inhibition of NO production might be useful for obtaining more differentiated hepatocytes in the process of in vitro induction of hepatocyte-specific differentiation. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)175-178
Number of pages4
JournalFEBS Letters
Volume474
Issue number2-3
DOIs
StatePublished - 2 Jun 2000

Keywords

  • Butyrate
  • Differentiation
  • Hepatocyte
  • Human
  • Nitric oxide
  • Urea

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