Augmentation of urea-synthetic capacity by inhibition of nitric oxide synthesis in butyrate-induced differentiated human hepatocytes

Jung Hwan Yoon; Hyo Suk Lee; Tae Hun Kim; Gwang Hoon Woo; Chung Yong Kim

doi:10.1016/S0014-5793(00)01599-4

Augmentation of urea-synthetic capacity by inhibition of nitric oxide synthesis in butyrate-induced differentiated human hepatocytes

Jung Hwan Yoon, Hyo Suk Lee, Tae Hun Kim, Gwang Hoon Woo, Chung Yong Kim

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

We have recently developed an in vitro differentiation model of immortalized non-transformed human hepatocytes using butyrate, and observed the induction of inducible NO synthase (iNOS). In this study, we analyzed the effect of NO on the urea-synthetic capacity of these cells. The inhibition of iNOS during butyrate treatment significantly increased the urea-synthetic capacity as compared to that of butyrate treatment alone, possibly through the further induction of ornithine transcarbamylase expression. Therefore, the inhibition of NO production might be useful for obtaining more differentiated hepatocytes in the process of in vitro induction of hepatocyte-specific differentiation. (C) 2000 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	175-178
Number of pages	4
Journal	FEBS Letters
Volume	474
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-5793(00)01599-4
State	Published - 2 Jun 2000

Bibliographical note

Funding Information:
The authors thank Ms. Hyejung Jung and Jinkyoung Jung for their excellent technical assistance. This work was supported by a grant from the Korean Foundation of Liver Research.

Keywords

Butyrate
Differentiation
Hepatocyte
Human
Nitric oxide
Urea

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10.1016/S0014-5793(00)01599-4

Cite this

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title = "Augmentation of urea-synthetic capacity by inhibition of nitric oxide synthesis in butyrate-induced differentiated human hepatocytes",

abstract = "We have recently developed an in vitro differentiation model of immortalized non-transformed human hepatocytes using butyrate, and observed the induction of inducible NO synthase (iNOS). In this study, we analyzed the effect of NO on the urea-synthetic capacity of these cells. The inhibition of iNOS during butyrate treatment significantly increased the urea-synthetic capacity as compared to that of butyrate treatment alone, possibly through the further induction of ornithine transcarbamylase expression. Therefore, the inhibition of NO production might be useful for obtaining more differentiated hepatocytes in the process of in vitro induction of hepatocyte-specific differentiation. (C) 2000 Federation of European Biochemical Societies.",

keywords = "Butyrate, Differentiation, Hepatocyte, Human, Nitric oxide, Urea",

author = "Yoon, {Jung Hwan} and Lee, {Hyo Suk} and Kim, {Tae Hun} and Woo, {Gwang Hoon} and Kim, {Chung Yong}",

note = "Funding Information: The authors thank Ms. Hyejung Jung and Jinkyoung Jung for their excellent technical assistance. This work was supported by a grant from the Korean Foundation of Liver Research. ",

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doi = "10.1016/S0014-5793(00)01599-4",

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T1 - Augmentation of urea-synthetic capacity by inhibition of nitric oxide synthesis in butyrate-induced differentiated human hepatocytes

AU - Yoon, Jung Hwan

AU - Lee, Hyo Suk

AU - Kim, Tae Hun

AU - Woo, Gwang Hoon

AU - Kim, Chung Yong

N1 - Funding Information: The authors thank Ms. Hyejung Jung and Jinkyoung Jung for their excellent technical assistance. This work was supported by a grant from the Korean Foundation of Liver Research.

PY - 2000/6/2

Y1 - 2000/6/2

N2 - We have recently developed an in vitro differentiation model of immortalized non-transformed human hepatocytes using butyrate, and observed the induction of inducible NO synthase (iNOS). In this study, we analyzed the effect of NO on the urea-synthetic capacity of these cells. The inhibition of iNOS during butyrate treatment significantly increased the urea-synthetic capacity as compared to that of butyrate treatment alone, possibly through the further induction of ornithine transcarbamylase expression. Therefore, the inhibition of NO production might be useful for obtaining more differentiated hepatocytes in the process of in vitro induction of hepatocyte-specific differentiation. (C) 2000 Federation of European Biochemical Societies.

AB - We have recently developed an in vitro differentiation model of immortalized non-transformed human hepatocytes using butyrate, and observed the induction of inducible NO synthase (iNOS). In this study, we analyzed the effect of NO on the urea-synthetic capacity of these cells. The inhibition of iNOS during butyrate treatment significantly increased the urea-synthetic capacity as compared to that of butyrate treatment alone, possibly through the further induction of ornithine transcarbamylase expression. Therefore, the inhibition of NO production might be useful for obtaining more differentiated hepatocytes in the process of in vitro induction of hepatocyte-specific differentiation. (C) 2000 Federation of European Biochemical Societies.

KW - Butyrate

KW - Differentiation

KW - Hepatocyte

KW - Human

KW - Nitric oxide

KW - Urea

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ER -

Augmentation of urea-synthetic capacity by inhibition of nitric oxide synthesis in butyrate-induced differentiated human hepatocytes

Abstract

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Keywords

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