Asymmetric sulfoxidation and amine binding by H64D/V68A and H64D/V68S Mb: Mechanistic insight into the chiral discrimination step

Shigeru Kato, Hui Jun Yang, Takafumi Ueno, Shin ichi Ozaki, George N. Phillips, Shunichi Fukuzumi, Yoshihito Watanabe

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

The H64D/V68A and H64D/V68S mutants of Myoglobin are found to oxidize thioanisole with high enantioselectivity and reactivity. These mutants are also capable of enantioselective binding of α-methylbenzylamine, which mimics an expected sulfoxidation intermediate. The kinetic study of the amine binding shows that the Fe-O bond cleavage in the intermediate may be the chiral discrimination step of the sulfoxidation.

Original languageEnglish
Pages (from-to)8506-8507
Number of pages2
JournalJournal of the American Chemical Society
Volume124
Issue number29
DOIs
StatePublished - 24 Jul 2002

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