Asticcacaulis solisilvae sp. nov., isolated from forest soil

An obligately aerobic, chemoheterotrophic, mesophilic prosthecate bacterium, designated strain CGM1-3EN^T, was isolated from the enrichment cultures of forest soil from Cheonggyesan Mountain, Republic of Korea. Cells were Gram-reaction-negative, motile rods (1.3-2.4 mm long by 0.30-0.75 mm wide) with single flagella. The strain grew at 10-37 °C (optimum 25-30 °C) and at pH 4.5-9.5 (optimum 5.0-7.0). The major cellular fatty acids were C_{16: 0}, C_{18: 1}ω7c 11- methyl, C_{12: 1} 3-OH and summed feature 8 (comprising C_{18: 1}ω7c/C_{18: 1}ω6c). The genomic DNA G+C content of strain CGM1-3EN^T was 63.7 mol%. The closest phylogenetic neighbour to strain CGM1-3EN^T was identified as Asticcacaulis biprosthecium DSM 4723^T (97.2% 16S rRNA gene sequence similarity) and the DNA-DNA hybridization value between strain CGM1- 3EN^T and A. biprosthecium DSM 4723^T was less than 24.5 %. Strain CGM1-3EN^T used Dglucose, D-fructose, sucrose, maltose, trehalose, D-mannose, D-mannitol, D-sorbitol, D-galactose, cellobiose, lactose, raffinose, fumarate, pyruvate, DL-alanine and glycerol as carbon sources. Based on data from the present polyphasic study, the forest soil isolate CGM1-3EN^T is considered to represent a novel species of the genus Asticcacaulis, for which the name Asticcacaulis solisilvae sp. nov. is proposed. The type strain is CGM1-3EN^T (=AIM0088^T=KCTC 32102^T=JCM 18544^T).