Association of the Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD (SLP-76) with the p85 subunit of phosphoinositide 3-kinase

Eun Kyung Shim; Chang Suk Moon; Gi Yeon Lee; Yun Jung Ha; Suhn Kee Chae; Jong Ran Lee

doi:10.1016/j.febslet.2004.07.090

Association of the Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD (SLP-76) with the p85 subunit of phosphoinositide 3-kinase

Eun Kyung Shim, Chang Suk Moon, Gi Yeon Lee, Yun Jung Ha, Suhn Kee Chae, Jong Ran Lee

Life Sciences

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

To investigate additional functions of the T cell adaptor, Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD (SLP-76), we performed a yeast two-hybrid assay using the N-terminal region of SLP-76 fused with the kinase domain of Syk. By screening a human leukemia cDNA library, we identified the p85 subunit of phosphoinositide 3-kinase (PI3K) as one of the interacting molecules. Unlike the SH2 domain of Vav or Nck, tyrosine phosphorylation of SLP-76 at position 113 or 128 was sufficient for it to associate with the N-terminal SH2 of p85. Collectively, these data suggest that SLP-76 may play a role in PI3K signaling pathways.

Original language	English
Pages (from-to)	35-40
Number of pages	6
Journal	FEBS Letters
Volume	575
Issue number	1-3
DOIs	https://doi.org/10.1016/j.febslet.2004.07.090
State	Published - 24 Sep 2004

Keywords

Ab, antibody
IB, immunoblotting
IP, immunoprecipitation
PI3K, phosphoinositide 3-kinase
PTK, protein tyrosine kinase
SH2, Src homology 2
SLP-76, Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD
TCR, T cell receptor
WT, wild-type

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10.1016/j.febslet.2004.07.090

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title = "Association of the Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD (SLP-76) with the p85 subunit of phosphoinositide 3-kinase",

abstract = "To investigate additional functions of the T cell adaptor, Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD (SLP-76), we performed a yeast two-hybrid assay using the N-terminal region of SLP-76 fused with the kinase domain of Syk. By screening a human leukemia cDNA library, we identified the p85 subunit of phosphoinositide 3-kinase (PI3K) as one of the interacting molecules. Unlike the SH2 domain of Vav or Nck, tyrosine phosphorylation of SLP-76 at position 113 or 128 was sufficient for it to associate with the N-terminal SH2 of p85. Collectively, these data suggest that SLP-76 may play a role in PI3K signaling pathways.",

keywords = "Ab, antibody, IB, immunoblotting, IP, immunoprecipitation, PI3K, phosphoinositide 3-kinase, PTK, protein tyrosine kinase, SH2, Src homology 2, SLP-76, Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD, TCR, T cell receptor, WT, wild-type",

author = "Shim, {Eun Kyung} and Moon, {Chang Suk} and Lee, {Gi Yeon} and Ha, {Yun Jung} and Chae, {Suhn Kee} and Lee, {Jong Ran}",

note = "Funding Information: We thank Drs. Alex Toker, Andr{\'e} Veillette, and Gary Koretzky for providing valuable reagents. This study was supported by a grant from the Korea Health 21 R&D project, Ministry of Health and Welfare, Republic of Korea (00-PJ1-PG3-21200-0045), and another from the Korea Science and Engineering Foundation through the Center for Cell Signaling Research at Ewha Womans University. E.K.S., J.Y.L., and Y.J.H. were supported in part by the Brain Korea 21 Program of the Korea Ministry of Education.",

year = "2004",

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doi = "10.1016/j.febslet.2004.07.090",

language = "English",

volume = "575",

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journal = "FEBS Letters",

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publisher = "Wiley-Blackwell",

number = "1-3",

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T1 - Association of the Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD (SLP-76) with the p85 subunit of phosphoinositide 3-kinase

AU - Shim, Eun Kyung

AU - Moon, Chang Suk

AU - Lee, Gi Yeon

AU - Ha, Yun Jung

AU - Chae, Suhn Kee

AU - Lee, Jong Ran

N1 - Funding Information: We thank Drs. Alex Toker, André Veillette, and Gary Koretzky for providing valuable reagents. This study was supported by a grant from the Korea Health 21 R&D project, Ministry of Health and Welfare, Republic of Korea (00-PJ1-PG3-21200-0045), and another from the Korea Science and Engineering Foundation through the Center for Cell Signaling Research at Ewha Womans University. E.K.S., J.Y.L., and Y.J.H. were supported in part by the Brain Korea 21 Program of the Korea Ministry of Education.

PY - 2004/9/24

Y1 - 2004/9/24

N2 - To investigate additional functions of the T cell adaptor, Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD (SLP-76), we performed a yeast two-hybrid assay using the N-terminal region of SLP-76 fused with the kinase domain of Syk. By screening a human leukemia cDNA library, we identified the p85 subunit of phosphoinositide 3-kinase (PI3K) as one of the interacting molecules. Unlike the SH2 domain of Vav or Nck, tyrosine phosphorylation of SLP-76 at position 113 or 128 was sufficient for it to associate with the N-terminal SH2 of p85. Collectively, these data suggest that SLP-76 may play a role in PI3K signaling pathways.

AB - To investigate additional functions of the T cell adaptor, Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD (SLP-76), we performed a yeast two-hybrid assay using the N-terminal region of SLP-76 fused with the kinase domain of Syk. By screening a human leukemia cDNA library, we identified the p85 subunit of phosphoinositide 3-kinase (PI3K) as one of the interacting molecules. Unlike the SH2 domain of Vav or Nck, tyrosine phosphorylation of SLP-76 at position 113 or 128 was sufficient for it to associate with the N-terminal SH2 of p85. Collectively, these data suggest that SLP-76 may play a role in PI3K signaling pathways.

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KW - IB, immunoblotting

KW - IP, immunoprecipitation

KW - PI3K, phosphoinositide 3-kinase

KW - PTK, protein tyrosine kinase

KW - SH2, Src homology 2

KW - SLP-76, Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD

KW - TCR, T cell receptor

KW - WT, wild-type

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U2 - 10.1016/j.febslet.2004.07.090

DO - 10.1016/j.febslet.2004.07.090

M3 - Article

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AN - SCOPUS:4544323522

SN - 0014-5793

VL - 575

SP - 35

EP - 40

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

Association of the Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD (SLP-76) with the p85 subunit of phosphoinositide 3-kinase

Abstract

Keywords

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