Association of the Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD (SLP-76) with the p85 subunit of phosphoinositide 3-kinase

Eun Kyung Shim; Chang Suk Moon; Gi Yeon Lee; Yun Jung Ha; Suhn Kee Chae; Jong Ran Lee

doi:10.1016/j.febslet.2004.07.090

Association of the Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD (SLP-76) with the p85 subunit of phosphoinositide 3-kinase

Eun Kyung Shim, Chang Suk Moon, Gi Yeon Lee, Yun Jung Ha, Suhn Kee Chae, Jong Ran Lee

Life Sciences

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

To investigate additional functions of the T cell adaptor, Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD (SLP-76), we performed a yeast two-hybrid assay using the N-terminal region of SLP-76 fused with the kinase domain of Syk. By screening a human leukemia cDNA library, we identified the p85 subunit of phosphoinositide 3-kinase (PI3K) as one of the interacting molecules. Unlike the SH2 domain of Vav or Nck, tyrosine phosphorylation of SLP-76 at position 113 or 128 was sufficient for it to associate with the N-terminal SH2 of p85. Collectively, these data suggest that SLP-76 may play a role in PI3K signaling pathways.

Original language	English
Pages (from-to)	35-40
Number of pages	6
Journal	FEBS Letters
Volume	575
Issue number	1-3
DOIs	https://doi.org/10.1016/j.febslet.2004.07.090
State	Published - 24 Sep 2004

Bibliographical note

Funding Information:
We thank Drs. Alex Toker, André Veillette, and Gary Koretzky for providing valuable reagents. This study was supported by a grant from the Korea Health 21 R&D project, Ministry of Health and Welfare, Republic of Korea (00-PJ1-PG3-21200-0045), and another from the Korea Science and Engineering Foundation through the Center for Cell Signaling Research at Ewha Womans University. E.K.S., J.Y.L., and Y.J.H. were supported in part by the Brain Korea 21 Program of the Korea Ministry of Education.

Keywords

Ab, antibody
IB, immunoblotting
IP, immunoprecipitation
PI3K, phosphoinositide 3-kinase
PTK, protein tyrosine kinase
SH2, Src homology 2
SLP-76, Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD
TCR, T cell receptor
WT, wild-type

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title = "Association of the Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD (SLP-76) with the p85 subunit of phosphoinositide 3-kinase",

abstract = "To investigate additional functions of the T cell adaptor, Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD (SLP-76), we performed a yeast two-hybrid assay using the N-terminal region of SLP-76 fused with the kinase domain of Syk. By screening a human leukemia cDNA library, we identified the p85 subunit of phosphoinositide 3-kinase (PI3K) as one of the interacting molecules. Unlike the SH2 domain of Vav or Nck, tyrosine phosphorylation of SLP-76 at position 113 or 128 was sufficient for it to associate with the N-terminal SH2 of p85. Collectively, these data suggest that SLP-76 may play a role in PI3K signaling pathways.",

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author = "Shim, {Eun Kyung} and Moon, {Chang Suk} and Lee, {Gi Yeon} and Ha, {Yun Jung} and Chae, {Suhn Kee} and Lee, {Jong Ran}",

note = "Funding Information: We thank Drs. Alex Toker, Andr{\'e} Veillette, and Gary Koretzky for providing valuable reagents. This study was supported by a grant from the Korea Health 21 R&D project, Ministry of Health and Welfare, Republic of Korea (00-PJ1-PG3-21200-0045), and another from the Korea Science and Engineering Foundation through the Center for Cell Signaling Research at Ewha Womans University. E.K.S., J.Y.L., and Y.J.H. were supported in part by the Brain Korea 21 Program of the Korea Ministry of Education.",

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language = "English",

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AU - Moon, Chang Suk

AU - Lee, Gi Yeon

AU - Ha, Yun Jung

AU - Chae, Suhn Kee

AU - Lee, Jong Ran

N1 - Funding Information: We thank Drs. Alex Toker, André Veillette, and Gary Koretzky for providing valuable reagents. This study was supported by a grant from the Korea Health 21 R&D project, Ministry of Health and Welfare, Republic of Korea (00-PJ1-PG3-21200-0045), and another from the Korea Science and Engineering Foundation through the Center for Cell Signaling Research at Ewha Womans University. E.K.S., J.Y.L., and Y.J.H. were supported in part by the Brain Korea 21 Program of the Korea Ministry of Education.

PY - 2004/9/24

Y1 - 2004/9/24

N2 - To investigate additional functions of the T cell adaptor, Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD (SLP-76), we performed a yeast two-hybrid assay using the N-terminal region of SLP-76 fused with the kinase domain of Syk. By screening a human leukemia cDNA library, we identified the p85 subunit of phosphoinositide 3-kinase (PI3K) as one of the interacting molecules. Unlike the SH2 domain of Vav or Nck, tyrosine phosphorylation of SLP-76 at position 113 or 128 was sufficient for it to associate with the N-terminal SH2 of p85. Collectively, these data suggest that SLP-76 may play a role in PI3K signaling pathways.

AB - To investigate additional functions of the T cell adaptor, Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD (SLP-76), we performed a yeast two-hybrid assay using the N-terminal region of SLP-76 fused with the kinase domain of Syk. By screening a human leukemia cDNA library, we identified the p85 subunit of phosphoinositide 3-kinase (PI3K) as one of the interacting molecules. Unlike the SH2 domain of Vav or Nck, tyrosine phosphorylation of SLP-76 at position 113 or 128 was sufficient for it to associate with the N-terminal SH2 of p85. Collectively, these data suggest that SLP-76 may play a role in PI3K signaling pathways.

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KW - SH2, Src homology 2

KW - SLP-76, Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD

KW - TCR, T cell receptor

KW - WT, wild-type

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ER -

Association of the Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD (SLP-76) with the p85 subunit of phosphoinositide 3-kinase

Abstract

Bibliographical note

Keywords

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