Association of irritability and anxiety with the neural mechanisms of implicit face emotion processing in youths with psychopathology

Joel Stoddard, Wan Ling Tseng, Pilyoung Kim, Gang Chen, Jennifer Yi, Laura Donahue, Melissa A. Brotman, Kenneth E. Towbin, Daniel S. Pine, Ellen Leibenluft

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67 Scopus citations


IMPORTANCE: Psychiatric comorbidity complicates clinical care and confounds efforts to elucidate the pathophysiology of commonly occurring symptoms in youths. To our knowledge, few studies have simultaneously assessed the effect of 2 continuously distributed traits on brain-behavior relationships in children with psychopathology. OBJECTIVE: To determine shared and unique effects of 2 major dimensions of child psychopathology, irritability and anxiety, on neural responses to facial emotions during functional magnetic resonance imaging. DESIGN, SETTING, AND PARTICIPANTS: Cross-sectional functional magnetic resonance imaging study in a large, well-characterized clinical sample at a research clinic at the National Institute of Mental Health. The referred sample included youths ages 8 to 17 years, 93 youths with anxiety, disruptive mood dysregulation, and/or attention-deficit/hyperactivity disorders and 22 healthy youths. MAIN OUTCOMES AND MEASURES: The child's irritability and anxiety were rated by both parent and child on the Affective Reactivity Index and Screen for Child Anxiety Related Disorders, respectively. Using functional magnetic resonance imaging, neural response was measured across the brain during gender labeling of varying intensities of angry, happy, or fearful face emotions. In mixed-effects analyses, the shared and unique effects of irritability and anxiety were tested on amygdala functional connectivity and activation to face emotions. RESULTS: The mean (SD) age of participants was 13.2 (2.6) years; of the 115 included, 64 were male. Irritability and/or anxiety influenced amygdala connectivity to the prefrontal and temporal cortex. Specifically, irritability and anxietyjointly influenced left amygdala to left medial prefrontal cortex connectivity during face emotion viewing (F4,888 = 9.20; P<.001 for mixed model term). During viewing of intensely angry faces, decreased connectivity was associated with high levels of both anxiety and irritability, whereas increased connectivity was associated with high levels of anxiety but low levels of irritability (Waldχ21 = 21.3; P <.001 for contrast). Irritability was associated with differences in neural response to face emotions in several areas (F2.888 ≥ 13.45; all P <.001). This primarily occurred in the ventral visual areas, with a positive association to angry and happy faces relative to fearful faces. CONCLUSIONS AND RELEVANCE: These data extend prior work conducted in youths with irritability or anxiety alone and suggest that research may miss important findings if the pathophysiology of irritability and anxiety are studied in isolation. Decreased amygdala-medial prefrontal cortex connectivity may mediate emotion dysregulation when very anxious and irritable youth process threat-related faces. Activation in the ventral visual circuitry suggests a mechanism through which signals of social approach (ie, happy and angry expressions) may capture attention in irritable youth.

Original languageEnglish
Pages (from-to)95-103
Number of pages9
JournalJAMA Psychiatry
Issue number1
StatePublished - 1 Jan 2017

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Copyright 2017 American Medical Association. All rights reserved.


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