TY - JOUR
T1 - Assessment of the purity of emodin by quantitative nuclear magnetic resonance spectroscopy and mass balance
AU - Park, Sojung
AU - Choi, Yu Jin
AU - Do, Giang Hoang
AU - Seo, Eun Kyoung
AU - Hyun, Seunghun
AU - Lee, Dongho
N1 - Funding Information:
This research was financially supported by the Ministry of Food and Drug Safety (15172MFDS189), Korea University, and the National Research Foundation of Korea (NRF-2019R1A2C1006226).
Publisher Copyright:
© 2019, Korean Society of Pharmacognosy. All rights reserved.
PY - 2019
Y1 - 2019
N2 - Quantitative nuclear magnetic resonance (qNMR) is a well-established method adopted by international pharmacopoeia for quantitative and purity analyses. Emodin is a type of anthraquinone, well known as the main active component of Fabaceae, Polygonaceae and Rhamnaceae. Purity analysis of emodin is usually performed by using the high-performance liquid chromatography (HPLC)-UV method. However, it cannot detect impurities such as salts, volatile matter, and trace elements. Using the qNMR method, it is possible to determine the compound content as well as the nature of the impurities. Several experimental parameters were optimized for the quantification, such as relaxation delay, spectral width, number of scans, temperature, pulse width, and acquisition time. The method was validated, and the results of the qNMR method were compared with those obtained by the HPLC and mass balance analysis methods. The qNMR method is specific, rapid, simple, and therefore, a valuable and reliable method for the purity analysis of emodin.
AB - Quantitative nuclear magnetic resonance (qNMR) is a well-established method adopted by international pharmacopoeia for quantitative and purity analyses. Emodin is a type of anthraquinone, well known as the main active component of Fabaceae, Polygonaceae and Rhamnaceae. Purity analysis of emodin is usually performed by using the high-performance liquid chromatography (HPLC)-UV method. However, it cannot detect impurities such as salts, volatile matter, and trace elements. Using the qNMR method, it is possible to determine the compound content as well as the nature of the impurities. Several experimental parameters were optimized for the quantification, such as relaxation delay, spectral width, number of scans, temperature, pulse width, and acquisition time. The method was validated, and the results of the qNMR method were compared with those obtained by the HPLC and mass balance analysis methods. The qNMR method is specific, rapid, simple, and therefore, a valuable and reliable method for the purity analysis of emodin.
KW - Emodin
KW - HPLC-UV
KW - Mass balance
KW - QNMR
UR - http://www.scopus.com/inward/record.url?scp=85075001241&partnerID=8YFLogxK
U2 - 10.20307/nps.2019.25.3.222
DO - 10.20307/nps.2019.25.3.222
M3 - Article
AN - SCOPUS:85075001241
SN - 1226-3907
VL - 25
SP - 222
EP - 227
JO - Natural Product Sciences
JF - Natural Product Sciences
IS - 3
ER -