Assessment of peripheral tissue perfusion by optical dynamic fluorescence imaging and nonlinear regression modeling

Yujung Kang, Jungsul Lee, Kihwan Kwon, Chulhee Choi

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Scopus citations


The purpose of this study is to examine the peripheral tissue perfusion rates by time-series analysis of distribution and elimination kinetics of a clinically proven NIR fluorescence probe, indocyanine green (ICG). We developed a new method, dynamic ICG perfusion imaging technique to evaluate peripheral tissue perfusion that employs planar imaging with a CCD digital imaging system and time-series analysis of the spatiotemporal dynamics (150s) of intravenously injected ICG by using nonlinear regression and differential evolution methods. Six parameters (α, β, s, d, m; parameters which depend on an arterial input function (AIF) into a lower extremity and p; perfusion rates in the lower extremity) were estimated by the nonlinear regression modeling method. We have confirmed the validity of our new method by applying the method to a normal control and a patient with peripheral arterial occlusion disease (PAOD). PAOD patient showed a unique AIF curve pattern, which was caused by collateral blood flow bypassing the occluded major artery. The lower extremity tissue perfusion rate of the PAOD patient was estimated as about 35% of those of normal values. These results indicate that ICG perfusion imaging method is sensitive enough to diagnose PAOD and capable of diagnosing functional arterial diseases.

Original languageEnglish
Title of host publicationPhotonic Therapeutics and Diagnostics VI
StatePublished - 2010
EventPhotonic Therapeutics and Diagnostics VI - San Francisco, CA, United States
Duration: 23 Jan 201025 Jan 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferencePhotonic Therapeutics and Diagnostics VI
Country/TerritoryUnited States
CitySan Francisco, CA


  • differential evolution
  • Indicator dilution curve
  • mathematical modeling
  • NIR fluorescence imaging
  • non-linear regression
  • peripheral arterial occlusive disease
  • tissue perfusion


Dive into the research topics of 'Assessment of peripheral tissue perfusion by optical dynamic fluorescence imaging and nonlinear regression modeling'. Together they form a unique fingerprint.

Cite this