Assessment of musculoskeletal infection in rats to determine usefulness of SPIO-enhanced MRI

Min Lee Sang, Sang Hoon Lee, Youn Kang Hae, Yon Baek Seung, Moon Kim Sung, Jin Shin Myung

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

OBJECTIVE. The objective of our study was to evaluate the usefulness of superparamagnetic iron oxide (SPIO)-enhanced MRI in experimental models of infectious disease and to analyze the intracellular uptake of SPIO. MATERIALS AND METHODS. Nine rats with infectious arthritis of the knee or soft-tissue infection were imaged on an MRI unit on days 4-6 after IV injection of a bacterial suspension. All animals were imaged on a T2-weighted fast spin-echo sequence before and 24 hours after administration of SPIO. The nine rats were classified into two groups according to the dose of SPIO. We calculated the relative signal-to-noise ratio (SNR) change and compared the relative SNR change with the histologic findings. We analyzed iron-loaded cells and the intracellular uptake of iron particles according to the dose of SPIO. RESULTS. The SNR value decreased in proportion to the increase in the number of iron-laden macrophages or fibroblasts in the wall of the soft-tissue abscess (p < 0.01). The intracellular uptake of iron particles was shown in fibroblasts as well as in macrophages, and their uptake in the fibroblasts was greater than that in the macrophages (p < 0.05). There was no statistically significant difference in the intracellular uptake of iron particles according to the dose of SPIO (p > 0.1). CONCLUSION. SPIO-enhanced MRI can be useful in evaluating infectious disease of the joint or soft tissue and is influenced by the uptake of iron particles in fibroblasts as well as macrophages.

Original languageEnglish
Pages (from-to)542-548
Number of pages7
JournalAmerican Journal of Roentgenology
Volume189
Issue number3
DOIs
StatePublished - Sep 2007

Keywords

  • Infectious disease
  • MRI
  • Musculoskeletal infection
  • Superparamagnetic iron oxide (SPIO)

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