Assessing effects of earthworm cast on methanotrophic community in a soil biocover by concurrent use of microarray and quantitative real-time PCR

Tae Gwan Kim, Kyung Eun Moon, Eun Hee Lee, Sun Ah Choi, Kyung Suk Cho

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19 Scopus citations

Abstract

Effects of earthworm cast (EC) on the methanotrophic community in a soil biocover were evaluated using microarray and quantitative real-time PCR (qRT-PCR). Soil was collected from a biocover with either soil alone or a mixture of soil and EC (3:1, w/w). The microarray results showed a more diverse methanotrophic community in the EC biocover than that in the soil biocover (p< 0.05). A principal component analysis result confirmed a substantial change in the methanotrophic community structure due to the added EC. Type I methanotrophs dominated both biocovers, with Methylobacter being most abundant. The qRT-PCR results showed that EC greatly increased the methanotrophic population levels (p< 0.05) up to 100 fold. In conclusion, EC can increase the population density of methanotrophs as well as their diversity, resulting in a substantial shift in the community structure. The results confirmed the promising potential of EC as a bed material for enhancing the biocover performance.

Original languageEnglish
Pages (from-to)52-55
Number of pages4
JournalApplied Soil Ecology
Volume50
Issue number1
DOIs
StatePublished - Oct 2011

Keywords

  • Earthworm cast
  • Methanotrophs
  • Microarray
  • Quantitative real-time PCR
  • Soil biocover

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