Assembly of collagen-binding peptide with collagen as a bioactive scaffold for osteogenesis in vitro and in vivo

Jue Yeon Lee, Jung Eun Choo, Young Suk Choi, Jun Bum Park, Do Sik Min, Seung Jin Lee, Hyung Keun Rhyu, In Ho Jo, Chong Pyoung Chung, Yoon Jeong Park

Research output: Contribution to journalArticlepeer-review

66 Scopus citations


Bioactive scaffolds inducing cell adhesion, differentiation have been premise for optimal formation of target tissue. Collagen has been employed as a tissue regenerative scaffold especially for bone regeneration and has been chemically surface-modified to present bioactivity. Herein, we show that peptide, denoted as collagen-binding motif (CBM, GLRSKSKKFRRPDIQYPDATDEDITSHM) identified from osteopontin (OPN) protein, was able to specifically bind collagen without chemical conjugation, while presenting apatite forming capability in vitro and in vivo. Collagen surface alone was not able to induce noticeable apatite nucleation however, mineralization was evident when assembled with CBM peptide, implying that the collagen-CBM assembly played a pivotal role in biomineralization. In vivo result further demonstrated that the CBM peptide in complex with material was able to induce bone formation by helping mineralization in the bone defect. Taken together, the CBM peptide herein and its assembly with collagen can be applied as an inducer of biomineralization as well as a bioactive scaffold for bone regeneration.

Original languageEnglish
Pages (from-to)4257-4267
Number of pages11
Issue number29
StatePublished - Oct 2007

Bibliographical note

Funding Information:
This study was supported in part by Korea Science and Technology Foundation (KOSEF) Nanobiotechnology development program (#2007-01317) entitled by Regenomics (Innovative surface activation of regenerative biomaterials) and in part by Engineering Research Center grant from KOSEF through Intelligent Biointerface Engineering Center (IBEC), Seoul National University, Korea.


  • Assembly
  • Bioactive scaffold
  • Biomineralization
  • Bone regeneration
  • Collagen-binding motif peptide
  • Osteopontin


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