Assembly of collagen-binding peptide with collagen as a bioactive scaffold for osteogenesis in vitro and in vivo

Jue Yeon Lee; Jung Eun Choo; Young Suk Choi; Jun Bum Park; Do Sik Min; Seung Jin Lee; Hyung Keun Rhyu; In Ho Jo; Chong Pyoung Chung; Yoon Jeong Park

doi:10.1016/j.biomaterials.2007.05.040

Assembly of collagen-binding peptide with collagen as a bioactive scaffold for osteogenesis in vitro and in vivo

Jue Yeon Lee, Jung Eun Choo, Young Suk Choi, Jun Bum Park, Do Sik Min, Seung Jin Lee, Hyung Keun Rhyu, In Ho Jo, Chong Pyoung Chung, Yoon Jeong Park

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

Bioactive scaffolds inducing cell adhesion, differentiation have been premise for optimal formation of target tissue. Collagen has been employed as a tissue regenerative scaffold especially for bone regeneration and has been chemically surface-modified to present bioactivity. Herein, we show that peptide, denoted as collagen-binding motif (CBM, GLRSKSKKFRRPDIQYPDATDEDITSHM) identified from osteopontin (OPN) protein, was able to specifically bind collagen without chemical conjugation, while presenting apatite forming capability in vitro and in vivo. Collagen surface alone was not able to induce noticeable apatite nucleation however, mineralization was evident when assembled with CBM peptide, implying that the collagen-CBM assembly played a pivotal role in biomineralization. In vivo result further demonstrated that the CBM peptide in complex with material was able to induce bone formation by helping mineralization in the bone defect. Taken together, the CBM peptide herein and its assembly with collagen can be applied as an inducer of biomineralization as well as a bioactive scaffold for bone regeneration.

Original language	English
Pages (from-to)	4257-4267
Number of pages	11
Journal	Biomaterials
Volume	28
Issue number	29
DOIs	https://doi.org/10.1016/j.biomaterials.2007.05.040
State	Published - Oct 2007

Bibliographical note

Funding Information:
This study was supported in part by Korea Science and Technology Foundation (KOSEF) Nanobiotechnology development program (#2007-01317) entitled by Regenomics (Innovative surface activation of regenerative biomaterials) and in part by Engineering Research Center grant from KOSEF through Intelligent Biointerface Engineering Center (IBEC), Seoul National University, Korea.

Keywords

Assembly
Bioactive scaffold
Biomineralization
Bone regeneration
Collagen-binding motif peptide
Osteopontin

Access to Document

10.1016/j.biomaterials.2007.05.040

Cite this

@article{16234647dfe649e6aa3ed72163388382,

title = "Assembly of collagen-binding peptide with collagen as a bioactive scaffold for osteogenesis in vitro and in vivo",

abstract = "Bioactive scaffolds inducing cell adhesion, differentiation have been premise for optimal formation of target tissue. Collagen has been employed as a tissue regenerative scaffold especially for bone regeneration and has been chemically surface-modified to present bioactivity. Herein, we show that peptide, denoted as collagen-binding motif (CBM, GLRSKSKKFRRPDIQYPDATDEDITSHM) identified from osteopontin (OPN) protein, was able to specifically bind collagen without chemical conjugation, while presenting apatite forming capability in vitro and in vivo. Collagen surface alone was not able to induce noticeable apatite nucleation however, mineralization was evident when assembled with CBM peptide, implying that the collagen-CBM assembly played a pivotal role in biomineralization. In vivo result further demonstrated that the CBM peptide in complex with material was able to induce bone formation by helping mineralization in the bone defect. Taken together, the CBM peptide herein and its assembly with collagen can be applied as an inducer of biomineralization as well as a bioactive scaffold for bone regeneration.",

keywords = "Assembly, Bioactive scaffold, Biomineralization, Bone regeneration, Collagen-binding motif peptide, Osteopontin",

author = "Lee, {Jue Yeon} and Choo, {Jung Eun} and Choi, {Young Suk} and Park, {Jun Bum} and Min, {Do Sik} and Lee, {Seung Jin} and Rhyu, {Hyung Keun} and Jo, {In Ho} and Chung, {Chong Pyoung} and Park, {Yoon Jeong}",

note = "Funding Information: This study was supported in part by Korea Science and Technology Foundation (KOSEF) Nanobiotechnology development program (#2007-01317) entitled by Regenomics (Innovative surface activation of regenerative biomaterials) and in part by Engineering Research Center grant from KOSEF through Intelligent Biointerface Engineering Center (IBEC), Seoul National University, Korea.",

year = "2007",

month = oct,

doi = "10.1016/j.biomaterials.2007.05.040",

language = "English",

volume = "28",

pages = "4257--4267",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier BV",

number = "29",

}

TY - JOUR

T1 - Assembly of collagen-binding peptide with collagen as a bioactive scaffold for osteogenesis in vitro and in vivo

AU - Lee, Jue Yeon

AU - Choo, Jung Eun

AU - Choi, Young Suk

AU - Park, Jun Bum

AU - Min, Do Sik

AU - Lee, Seung Jin

AU - Rhyu, Hyung Keun

AU - Jo, In Ho

AU - Chung, Chong Pyoung

AU - Park, Yoon Jeong

N1 - Funding Information: This study was supported in part by Korea Science and Technology Foundation (KOSEF) Nanobiotechnology development program (#2007-01317) entitled by Regenomics (Innovative surface activation of regenerative biomaterials) and in part by Engineering Research Center grant from KOSEF through Intelligent Biointerface Engineering Center (IBEC), Seoul National University, Korea.

PY - 2007/10

Y1 - 2007/10

N2 - Bioactive scaffolds inducing cell adhesion, differentiation have been premise for optimal formation of target tissue. Collagen has been employed as a tissue regenerative scaffold especially for bone regeneration and has been chemically surface-modified to present bioactivity. Herein, we show that peptide, denoted as collagen-binding motif (CBM, GLRSKSKKFRRPDIQYPDATDEDITSHM) identified from osteopontin (OPN) protein, was able to specifically bind collagen without chemical conjugation, while presenting apatite forming capability in vitro and in vivo. Collagen surface alone was not able to induce noticeable apatite nucleation however, mineralization was evident when assembled with CBM peptide, implying that the collagen-CBM assembly played a pivotal role in biomineralization. In vivo result further demonstrated that the CBM peptide in complex with material was able to induce bone formation by helping mineralization in the bone defect. Taken together, the CBM peptide herein and its assembly with collagen can be applied as an inducer of biomineralization as well as a bioactive scaffold for bone regeneration.

AB - Bioactive scaffolds inducing cell adhesion, differentiation have been premise for optimal formation of target tissue. Collagen has been employed as a tissue regenerative scaffold especially for bone regeneration and has been chemically surface-modified to present bioactivity. Herein, we show that peptide, denoted as collagen-binding motif (CBM, GLRSKSKKFRRPDIQYPDATDEDITSHM) identified from osteopontin (OPN) protein, was able to specifically bind collagen without chemical conjugation, while presenting apatite forming capability in vitro and in vivo. Collagen surface alone was not able to induce noticeable apatite nucleation however, mineralization was evident when assembled with CBM peptide, implying that the collagen-CBM assembly played a pivotal role in biomineralization. In vivo result further demonstrated that the CBM peptide in complex with material was able to induce bone formation by helping mineralization in the bone defect. Taken together, the CBM peptide herein and its assembly with collagen can be applied as an inducer of biomineralization as well as a bioactive scaffold for bone regeneration.

KW - Assembly

KW - Bioactive scaffold

KW - Biomineralization

KW - Bone regeneration

KW - Collagen-binding motif peptide

KW - Osteopontin

UR - http://www.scopus.com/inward/record.url?scp=34547681862&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2007.05.040

DO - 10.1016/j.biomaterials.2007.05.040

M3 - Article

C2 - 17604098

AN - SCOPUS:34547681862

SN - 0142-9612

VL - 28

SP - 4257

EP - 4267

JO - Biomaterials

JF - Biomaterials

IS - 29

ER -

Assembly of collagen-binding peptide with collagen as a bioactive scaffold for osteogenesis in vitro and in vivo

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this