Abstract
Alternative splicing is in important mechanism of modulating gene function and expression which greatly expands transcriptome diversity. ASmodeler is a novel web-based utility that finds gene models including alternative splicing events from genomic alignment of mRNA, EST and protein sequences. User-supplied sequences are aligned against the genome map using the BLAT and SIM4 programs. Resulting exon connectivity is analyzed by applying graph-theoretic methods to build all possible gene models including splice variants. The algorithm essentially combines the genome-based sequence clustering and transcript assembly procedures in a coherent fashion. In addition to the user-supplied sequences, UniGene clusters and many well-known gene predictions such as Genscan, Ensembl and Acembly may be included in gene modeling. The current implementation supports human, mouse and rat genomes. ASmodeler is available at http://genome.ewha.ac.kr/ECgene/ASmodeler/.
| Original language | English |
|---|---|
| Pages (from-to) | W181-W186 |
| Journal | Nucleic Acids Research |
| Volume | 32 |
| Issue number | WEB SERVER ISS. |
| DOIs | |
| State | Published - 1 Jul 2004 |
Bibliographical note
Funding Information:We are grateful to the UCSC Genome Center for making such wonderful resources available to the public. This work was supported by the Ministry of Science and Technology of Korea through the bioinformatics research program of MOST NRDP (M1-0217-00-0027) and the Korea Science and Engineering Foundation through the Center for Cell Signaling Research at Ewha Womans University.