Artificial Rod and Cone Photoreceptors with Human-Like Spectral Sensitivities

Byeongho Park; Heehong Yang; Tai Hwan Ha; Hyun Seo Park; Seung Ja Oh; Yong Sang Ryu; Youngho Cho; Hyo Suk Kim; Juyeong Oh; Dong Kyu Lee; Chulki Kim; Taikjin Lee; Minah Seo; Jaebin Choi; Young Min Jhon; Deok Ha Woo; Seok Lee; Seok Hwan Kim; Hyuk Jae Lee; Seong Chan Jun; Hyun Seok Song; Tai Hyun Park; Jae Hun Kim

doi:10.1002/adma.201706764

Artificial Rod and Cone Photoreceptors with Human-Like Spectral Sensitivities

Byeongho Park, Heehong Yang, Tai Hwan Ha, Hyun Seo Park, Seung Ja Oh, Yong Sang Ryu, Youngho Cho, Hyo Suk Kim, Juyeong Oh, Dong Kyu Lee, Chulki Kim, Taikjin Lee, Minah Seo, Jaebin Choi, Young Min Jhon, Deok Ha Woo, Seok Lee, Seok Hwan Kim, Hyuk Jae Lee, Seong Chan JunHyun Seok Song, Tai Hyun Park, Jae Hun Kim

Department of Nutritional Science & Food Management

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Photosensitive materials contain biologically engineered elements and are constructed using delicate techniques, with special attention devoted to efficiency, stability, and biocompatibility. However, to date, no photosensitive material has been developed to replace damaged visual-systems to detect light and transmit the signal to a neuron in the human body. In the current study, artificial nanovesicle-based photosensitive materials are observed to possess the characteristics of photoreceptors similar to the human eye. The materials exhibit considerably effective spectral characteristics according to each pigment. Four photoreceptors originating from the human eye with color-distinguishability are produced in human embryonic kidney (HEK)-293 cells and partially purified in the form of nanovesicles. Under various wavelengths of visible light, electrochemical measurements are performed to analyze the physiological behavior and kinetics of the photoreceptors, with graphene, performing as an electrode, playing an important role in the lipid bilayer deposition and oxygen reduction processes. Four nanovesicles with different photoreceptors, namely, rhodopsin (Rho), short-, medium-, and longwave sensitive opsin 1 (1SW, 1MW, 1LW), show remarkable color-dependent characteristics, consistent with those of natural human retina. With four different light-emitting diodes for functional verification, the photoreceptors embedded in nanovesicles show remarkably specific color sensitivity. This study demonstrates the potential applications of light-activated platforms in biological optoelectronic industries.

Original language	English
Article number	1706764
Journal	Advanced Materials
Volume	30
Issue number	27
DOIs	https://doi.org/10.1002/adma.201706764
State	Published - 5 Jul 2018

Bibliographical note

Funding Information:
B.P. and H.Y. contributed equally to this work. This research was supported by the National Research Foundation funded by the Korean government (MSIT) (No. NRF-2018R1A2B3004498). This project received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (Grant Agreement No. 682286). This study was also supported by KIST Institutional Program (No. 2E28390), Samsung Research Funding Center of Samsung Electronics under Project Number SRFC-IT1701-02, Korea Basic Science Institute (No. D38410), and a National Research Council of Science & Technology (NST) grant by the Korea government (MSIP) (No. CRC-16-01-KRICT).

Funding Information:
B.P. and H.Y. contributed equally to this work. This research was supported by the National Research Foundation funded by the Korean government (MSIT) (No. NRF-2018R1A2B3004498). This project received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (Grant Agreement No. 682286). This study was also supported by KIST Institutional Program (No. 2E28390), Samsung Research Funding Center of Samsung Electronics under Project Number SRFC-IT1701-02, Korea Basic Science Institute (No. D38410), and a National Research Council of Science & Technology (NST) grant by the Korea government (MSIP) (No. CRC-16-01-KRICT).

Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Keywords

artificial biomaterials
color sensitivity
graphene
photoreceptors
spectral characteristics

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10.1002/adma.201706764

Cite this

Park, B., Yang, H., Ha, T. H., Park, H. S., Oh, S. J., Ryu, Y. S., Cho, Y., Kim, H. S., Oh, J., Lee, D. K., Kim, C., Lee, T., Seo, M., Choi, J., Jhon, Y. M., Woo, D. H., Lee, S., Kim, S. H., Lee, H. J., ... Kim, J. H. (2018). Artificial Rod and Cone Photoreceptors with Human-Like Spectral Sensitivities. Advanced Materials, 30(27), Article 1706764. https://doi.org/10.1002/adma.201706764

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title = "Artificial Rod and Cone Photoreceptors with Human-Like Spectral Sensitivities",

abstract = "Photosensitive materials contain biologically engineered elements and are constructed using delicate techniques, with special attention devoted to efficiency, stability, and biocompatibility. However, to date, no photosensitive material has been developed to replace damaged visual-systems to detect light and transmit the signal to a neuron in the human body. In the current study, artificial nanovesicle-based photosensitive materials are observed to possess the characteristics of photoreceptors similar to the human eye. The materials exhibit considerably effective spectral characteristics according to each pigment. Four photoreceptors originating from the human eye with color-distinguishability are produced in human embryonic kidney (HEK)-293 cells and partially purified in the form of nanovesicles. Under various wavelengths of visible light, electrochemical measurements are performed to analyze the physiological behavior and kinetics of the photoreceptors, with graphene, performing as an electrode, playing an important role in the lipid bilayer deposition and oxygen reduction processes. Four nanovesicles with different photoreceptors, namely, rhodopsin (Rho), short-, medium-, and longwave sensitive opsin 1 (1SW, 1MW, 1LW), show remarkable color-dependent characteristics, consistent with those of natural human retina. With four different light-emitting diodes for functional verification, the photoreceptors embedded in nanovesicles show remarkably specific color sensitivity. This study demonstrates the potential applications of light-activated platforms in biological optoelectronic industries.",

keywords = "artificial biomaterials, color sensitivity, graphene, photoreceptors, spectral characteristics",

author = "Byeongho Park and Heehong Yang and Ha, {Tai Hwan} and Park, {Hyun Seo} and Oh, {Seung Ja} and Ryu, {Yong Sang} and Youngho Cho and Kim, {Hyo Suk} and Juyeong Oh and Lee, {Dong Kyu} and Chulki Kim and Taikjin Lee and Minah Seo and Jaebin Choi and Jhon, {Young Min} and Woo, {Deok Ha} and Seok Lee and Kim, {Seok Hwan} and Lee, {Hyuk Jae} and Jun, {Seong Chan} and Song, {Hyun Seok} and Park, {Tai Hyun} and Kim, {Jae Hun}",

note = "Funding Information: B.P. and H.Y. contributed equally to this work. This research was supported by the National Research Foundation funded by the Korean government (MSIT) (No. NRF-2018R1A2B3004498). This project received funding from the European Research Council (ERC) under the European Union{\textquoteright}s Horizon 2020 research and innovation program (Grant Agreement No. 682286). This study was also supported by KIST Institutional Program (No. 2E28390), Samsung Research Funding Center of Samsung Electronics under Project Number SRFC-IT1701-02, Korea Basic Science Institute (No. D38410), and a National Research Council of Science & Technology (NST) grant by the Korea government (MSIP) (No. CRC-16-01-KRICT). Funding Information: B.P. and H.Y. contributed equally to this work. This research was supported by the National Research Foundation funded by the Korean government (MSIT) (No. NRF-2018R1A2B3004498). This project received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (Grant Agreement No. 682286). This study was also supported by KIST Institutional Program (No. 2E28390), Samsung Research Funding Center of Samsung Electronics under Project Number SRFC-IT1701-02, Korea Basic Science Institute (No. D38410), and a National Research Council of Science & Technology (NST) grant by the Korea government (MSIP) (No. CRC-16-01-KRICT). Publisher Copyright: {\textcopyright} 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",

year = "2018",

month = jul,

day = "5",

doi = "10.1002/adma.201706764",

language = "English",

volume = "30",

journal = "Advanced Materials",

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Park, B, Yang, H, Ha, TH, Park, HS, Oh, SJ, Ryu, YS, Cho, Y, Kim, HS, Oh, J, Lee, DK, Kim, C, Lee, T, Seo, M, Choi, J, Jhon, YM, Woo, DH, Lee, S, Kim, SH, Lee, HJ, Jun, SC, Song, HS, Park, TH & Kim, JH 2018, 'Artificial Rod and Cone Photoreceptors with Human-Like Spectral Sensitivities', Advanced Materials, vol. 30, no. 27, 1706764. https://doi.org/10.1002/adma.201706764

TY - JOUR

T1 - Artificial Rod and Cone Photoreceptors with Human-Like Spectral Sensitivities

AU - Park, Byeongho

AU - Yang, Heehong

AU - Ha, Tai Hwan

AU - Park, Hyun Seo

AU - Oh, Seung Ja

AU - Ryu, Yong Sang

AU - Cho, Youngho

AU - Kim, Hyo Suk

AU - Oh, Juyeong

AU - Lee, Dong Kyu

AU - Kim, Chulki

AU - Lee, Taikjin

AU - Seo, Minah

AU - Choi, Jaebin

AU - Jhon, Young Min

AU - Woo, Deok Ha

AU - Lee, Seok

AU - Kim, Seok Hwan

AU - Lee, Hyuk Jae

AU - Jun, Seong Chan

AU - Song, Hyun Seok

AU - Park, Tai Hyun

AU - Kim, Jae Hun

N1 - Funding Information: B.P. and H.Y. contributed equally to this work. This research was supported by the National Research Foundation funded by the Korean government (MSIT) (No. NRF-2018R1A2B3004498). This project received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (Grant Agreement No. 682286). This study was also supported by KIST Institutional Program (No. 2E28390), Samsung Research Funding Center of Samsung Electronics under Project Number SRFC-IT1701-02, Korea Basic Science Institute (No. D38410), and a National Research Council of Science & Technology (NST) grant by the Korea government (MSIP) (No. CRC-16-01-KRICT). Funding Information: B.P. and H.Y. contributed equally to this work. This research was supported by the National Research Foundation funded by the Korean government (MSIT) (No. NRF-2018R1A2B3004498). This project received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (Grant Agreement No. 682286). This study was also supported by KIST Institutional Program (No. 2E28390), Samsung Research Funding Center of Samsung Electronics under Project Number SRFC-IT1701-02, Korea Basic Science Institute (No. D38410), and a National Research Council of Science & Technology (NST) grant by the Korea government (MSIP) (No. CRC-16-01-KRICT). Publisher Copyright: © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

PY - 2018/7/5

Y1 - 2018/7/5

N2 - Photosensitive materials contain biologically engineered elements and are constructed using delicate techniques, with special attention devoted to efficiency, stability, and biocompatibility. However, to date, no photosensitive material has been developed to replace damaged visual-systems to detect light and transmit the signal to a neuron in the human body. In the current study, artificial nanovesicle-based photosensitive materials are observed to possess the characteristics of photoreceptors similar to the human eye. The materials exhibit considerably effective spectral characteristics according to each pigment. Four photoreceptors originating from the human eye with color-distinguishability are produced in human embryonic kidney (HEK)-293 cells and partially purified in the form of nanovesicles. Under various wavelengths of visible light, electrochemical measurements are performed to analyze the physiological behavior and kinetics of the photoreceptors, with graphene, performing as an electrode, playing an important role in the lipid bilayer deposition and oxygen reduction processes. Four nanovesicles with different photoreceptors, namely, rhodopsin (Rho), short-, medium-, and longwave sensitive opsin 1 (1SW, 1MW, 1LW), show remarkable color-dependent characteristics, consistent with those of natural human retina. With four different light-emitting diodes for functional verification, the photoreceptors embedded in nanovesicles show remarkably specific color sensitivity. This study demonstrates the potential applications of light-activated platforms in biological optoelectronic industries.

AB - Photosensitive materials contain biologically engineered elements and are constructed using delicate techniques, with special attention devoted to efficiency, stability, and biocompatibility. However, to date, no photosensitive material has been developed to replace damaged visual-systems to detect light and transmit the signal to a neuron in the human body. In the current study, artificial nanovesicle-based photosensitive materials are observed to possess the characteristics of photoreceptors similar to the human eye. The materials exhibit considerably effective spectral characteristics according to each pigment. Four photoreceptors originating from the human eye with color-distinguishability are produced in human embryonic kidney (HEK)-293 cells and partially purified in the form of nanovesicles. Under various wavelengths of visible light, electrochemical measurements are performed to analyze the physiological behavior and kinetics of the photoreceptors, with graphene, performing as an electrode, playing an important role in the lipid bilayer deposition and oxygen reduction processes. Four nanovesicles with different photoreceptors, namely, rhodopsin (Rho), short-, medium-, and longwave sensitive opsin 1 (1SW, 1MW, 1LW), show remarkable color-dependent characteristics, consistent with those of natural human retina. With four different light-emitting diodes for functional verification, the photoreceptors embedded in nanovesicles show remarkably specific color sensitivity. This study demonstrates the potential applications of light-activated platforms in biological optoelectronic industries.

KW - artificial biomaterials

KW - color sensitivity

KW - graphene

KW - photoreceptors

KW - spectral characteristics

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DO - 10.1002/adma.201706764

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VL - 30

JO - Advanced Materials

JF - Advanced Materials

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M1 - 1706764

ER -

Artificial Rod and Cone Photoreceptors with Human-Like Spectral Sensitivities

Abstract

Bibliographical note

Keywords

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