Arsenic trioxide inhibits cell growth in SH-SY5Y and SK-N-AS neuroblastoma cell lines by a different mechanism

So Youn Woo, Mi Young Lee, Yun Jae Jung, Eun Sun Yoo, Ju Young Seoh, Hee Young Shin, Hyo Seop Ahn, Kyung Ha Ryu

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21 Scopus citations


Neuroblastoma, characterized by heterogeneous cell population, is a common solid tumor in childhood and some malignant neuroblastomas are refractory to conventional chemotherapy. Recently, treatment with arsenic trioxide (As 2 O 3 ) was found effective in the treatment of acute promyelocytic leukemia as well as neuroblastoma cells by inducing apoptosis. To define the mechanism contributing to cell death in those heterogenous cell populations, the authors used two different types of neuroblastoma cells, SH-SY5Y and SK-N-AS, to compare the pathways that mediate death response to arsenic trioxide. With arsenic trioxide exposure, both cell lines were arrested at the S-G2/M phase with the increase of cyclin B expression and CDK1 activity. Although caspase 3 was activated in both cell lines, the NF-?B activity and the expression of cyclin D1, cyclin E, and p27 were different. Therefore, arsenic trioxide could be an effective cytotoxic drug for the treatment of heterogeneous cellular population of neuroblastoma.

Original languageEnglish
Pages (from-to)231-243
Number of pages13
JournalPediatric Hematology and Oncology
Issue number3
StatePublished - Apr 2006

Bibliographical note

Funding Information:
Received 11 August 2005; accepted 2 December 2005. This work was supported by a Korea Research Foundation Grant (KRF-97-F00018). Address correspondence to Kyung-Ha Ryu, MD, PhD, Associate Professor, Department of Pediatrics, College of Medicine, Ewha Woman’s University, Seoul 158-710, Korea. E-mail:


  • Apoptosis
  • Arsenic trioxide
  • Cell cycle
  • Neuroblastoma


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