Arsenic trioxide inhibits cell growth in SH-SY5Y and SK-N-AS neuroblastoma cell lines by a different mechanism

So Youn Woo; Mi Young Lee; Yun Jae Jung; Eun Sun Yoo; Ju Young Seoh; Hee Young Shin; Hyo Seop Ahn; Kyung Ha Ryu

doi:10.1080/08880010500506818

Arsenic trioxide inhibits cell growth in SH-SY5Y and SK-N-AS neuroblastoma cell lines by a different mechanism

So Youn Woo, Mi Young Lee, Yun Jae Jung, Eun Sun Yoo, Ju Young Seoh, Hee Young Shin, Hyo Seop Ahn, Kyung Ha Ryu

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Neuroblastoma, characterized by heterogeneous cell population, is a common solid tumor in childhood and some malignant neuroblastomas are refractory to conventional chemotherapy. Recently, treatment with arsenic trioxide (As 2 O 3 ) was found effective in the treatment of acute promyelocytic leukemia as well as neuroblastoma cells by inducing apoptosis. To define the mechanism contributing to cell death in those heterogenous cell populations, the authors used two different types of neuroblastoma cells, SH-SY5Y and SK-N-AS, to compare the pathways that mediate death response to arsenic trioxide. With arsenic trioxide exposure, both cell lines were arrested at the S-G2/M phase with the increase of cyclin B expression and CDK1 activity. Although caspase 3 was activated in both cell lines, the NF-?B activity and the expression of cyclin D1, cyclin E, and p27 were different. Therefore, arsenic trioxide could be an effective cytotoxic drug for the treatment of heterogeneous cellular population of neuroblastoma.

Original language	English
Pages (from-to)	231-243
Number of pages	13
Journal	Pediatric Hematology and Oncology
Volume	23
Issue number	3
DOIs	https://doi.org/10.1080/08880010500506818
State	Published - Apr 2006

Keywords

Apoptosis
Arsenic trioxide
Cell cycle
Neuroblastoma

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title = "Arsenic trioxide inhibits cell growth in SH-SY5Y and SK-N-AS neuroblastoma cell lines by a different mechanism",

abstract = "Neuroblastoma, characterized by heterogeneous cell population, is a common solid tumor in childhood and some malignant neuroblastomas are refractory to conventional chemotherapy. Recently, treatment with arsenic trioxide (As 2 O 3 ) was found effective in the treatment of acute promyelocytic leukemia as well as neuroblastoma cells by inducing apoptosis. To define the mechanism contributing to cell death in those heterogenous cell populations, the authors used two different types of neuroblastoma cells, SH-SY5Y and SK-N-AS, to compare the pathways that mediate death response to arsenic trioxide. With arsenic trioxide exposure, both cell lines were arrested at the S-G2/M phase with the increase of cyclin B expression and CDK1 activity. Although caspase 3 was activated in both cell lines, the NF-?B activity and the expression of cyclin D1, cyclin E, and p27 were different. Therefore, arsenic trioxide could be an effective cytotoxic drug for the treatment of heterogeneous cellular population of neuroblastoma.",

keywords = "Apoptosis, Arsenic trioxide, Cell cycle, Neuroblastoma",

author = "Woo, {So Youn} and Lee, {Mi Young} and Jung, {Yun Jae} and Yoo, {Eun Sun} and Seoh, {Ju Young} and Shin, {Hee Young} and Ahn, {Hyo Seop} and Ryu, {Kyung Ha}",

note = "Funding Information: Received 11 August 2005; accepted 2 December 2005. This work was supported by a Korea Research Foundation Grant (KRF-97-F00018). Address correspondence to Kyung-Ha Ryu, MD, PhD, Associate Professor, Department of Pediatrics, College of Medicine, Ewha Woman{\textquoteright}s University, Seoul 158-710, Korea. E-mail: ykh@ewha.ac.kr",

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doi = "10.1080/08880010500506818",

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AU - Woo, So Youn

AU - Lee, Mi Young

AU - Jung, Yun Jae

AU - Yoo, Eun Sun

AU - Seoh, Ju Young

AU - Shin, Hee Young

AU - Ahn, Hyo Seop

AU - Ryu, Kyung Ha

N1 - Funding Information: Received 11 August 2005; accepted 2 December 2005. This work was supported by a Korea Research Foundation Grant (KRF-97-F00018). Address correspondence to Kyung-Ha Ryu, MD, PhD, Associate Professor, Department of Pediatrics, College of Medicine, Ewha Woman’s University, Seoul 158-710, Korea. E-mail: ykh@ewha.ac.kr

PY - 2006/4

Y1 - 2006/4

N2 - Neuroblastoma, characterized by heterogeneous cell population, is a common solid tumor in childhood and some malignant neuroblastomas are refractory to conventional chemotherapy. Recently, treatment with arsenic trioxide (As 2 O 3 ) was found effective in the treatment of acute promyelocytic leukemia as well as neuroblastoma cells by inducing apoptosis. To define the mechanism contributing to cell death in those heterogenous cell populations, the authors used two different types of neuroblastoma cells, SH-SY5Y and SK-N-AS, to compare the pathways that mediate death response to arsenic trioxide. With arsenic trioxide exposure, both cell lines were arrested at the S-G2/M phase with the increase of cyclin B expression and CDK1 activity. Although caspase 3 was activated in both cell lines, the NF-?B activity and the expression of cyclin D1, cyclin E, and p27 were different. Therefore, arsenic trioxide could be an effective cytotoxic drug for the treatment of heterogeneous cellular population of neuroblastoma.

AB - Neuroblastoma, characterized by heterogeneous cell population, is a common solid tumor in childhood and some malignant neuroblastomas are refractory to conventional chemotherapy. Recently, treatment with arsenic trioxide (As 2 O 3 ) was found effective in the treatment of acute promyelocytic leukemia as well as neuroblastoma cells by inducing apoptosis. To define the mechanism contributing to cell death in those heterogenous cell populations, the authors used two different types of neuroblastoma cells, SH-SY5Y and SK-N-AS, to compare the pathways that mediate death response to arsenic trioxide. With arsenic trioxide exposure, both cell lines were arrested at the S-G2/M phase with the increase of cyclin B expression and CDK1 activity. Although caspase 3 was activated in both cell lines, the NF-?B activity and the expression of cyclin D1, cyclin E, and p27 were different. Therefore, arsenic trioxide could be an effective cytotoxic drug for the treatment of heterogeneous cellular population of neuroblastoma.

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Arsenic trioxide inhibits cell growth in SH-SY5Y and SK-N-AS neuroblastoma cell lines by a different mechanism

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Keywords

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