Abstract
Neuroblastoma, characterized by heterogeneous cell population, is a common solid tumor in childhood and some malignant neuroblastomas are refractory to conventional chemotherapy. Recently, treatment with arsenic trioxide (As 2 O 3 ) was found effective in the treatment of acute promyelocytic leukemia as well as neuroblastoma cells by inducing apoptosis. To define the mechanism contributing to cell death in those heterogenous cell populations, the authors used two different types of neuroblastoma cells, SH-SY5Y and SK-N-AS, to compare the pathways that mediate death response to arsenic trioxide. With arsenic trioxide exposure, both cell lines were arrested at the S-G2/M phase with the increase of cyclin B expression and CDK1 activity. Although caspase 3 was activated in both cell lines, the NF-?B activity and the expression of cyclin D1, cyclin E, and p27 were different. Therefore, arsenic trioxide could be an effective cytotoxic drug for the treatment of heterogeneous cellular population of neuroblastoma.
Original language | English |
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Pages (from-to) | 231-243 |
Number of pages | 13 |
Journal | Pediatric Hematology and Oncology |
Volume | 23 |
Issue number | 3 |
DOIs | |
State | Published - Apr 2006 |
Bibliographical note
Funding Information:Received 11 August 2005; accepted 2 December 2005. This work was supported by a Korea Research Foundation Grant (KRF-97-F00018). Address correspondence to Kyung-Ha Ryu, MD, PhD, Associate Professor, Department of Pediatrics, College of Medicine, Ewha Woman’s University, Seoul 158-710, Korea. E-mail: [email protected]
Keywords
- Apoptosis
- Arsenic trioxide
- Cell cycle
- Neuroblastoma