AP-1-targeting anti-inflammatory activity of the methanolic extract of Persicaria Chinensis

Muhammad Jahangir Hossen, Seung Cheol Kim, Young Jin Son, Kwang Soo Baek, Eunji Kim, Woo Seok Yang, Deok Jeong, Jae Gwang Park, Han Gyung Kim, Woo Jae Chung, Keejung Yoon, Chongsuk Ryou, Sang Yeol Lee, Jong Hoon Kim, Jae Youl Cho

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

In traditional Chinese medicine, Persicaria chinensis L. has been prescribed to cure numerous inflammatory disorders. We previously analyzed the bioactivity of the methanol extract of this plant (Pc-ME) against LPS-induced NO and PGE2 in RAW264.7 macrophages and found that it prevented HCl/EtOH-induced gastric ulcers in mice. The purpose of the current study was to explore the molecular mechanism by which Pc-ME inhibits activator protein-(AP-) 1 activation pathway and mediates its hepatoprotective activity. To investigate the putative therapeutic properties of Pc-ME against AP-1-mediated inflammation and hepatotoxicity, lipopolysaccharide-(LPS-) stimulated RAW264.7 and U937 cells, a monocyte-like human cell line, and an LPS/D-galactosamine-(D-GalN-) induced acute hepatitis mouse model were employed. The expression of LPS-induced proinflammatory cytokines including interleukin-(IL-) 1β, IL-6, and tumor necrosis factor-α (TNF-α) was significantly diminished by Pc-ME. Moreover, Pc-ME reduced AP-1 activation and mitogen-activated protein kinase (MAPK) phosphorylation in both LPS-stimulated RAW264.7 cells and differentiated U937 cells. Additionally, we highlighted the hepatoprotective and curative effects of Pc-ME pretreated orally in a mouse model of LPS/D-GalN-intoxicated acute liver injury by demonstrating the significant reduction in elevated serum AST and ALT levels and histological damage. Therefore, these results strongly suggest that Pc-ME could function as an antihepatitis remedy suppressing MAPK/AP-1-mediated inflammatory events.

Original languageEnglish
Article number608126
JournalEvidence-based Complementary and Alternative Medicine
Volume2015
DOIs
StatePublished - 2015

Fingerprint

Dive into the research topics of 'AP-1-targeting anti-inflammatory activity of the methanolic extract of Persicaria Chinensis'. Together they form a unique fingerprint.

Cite this