Anti-pigmentary effect of (-)-4-hydroxysattabacin from the marine-derived bacterium Bacillus sp.

Kyuri Kim, Alain S. Leutou, Haein Jeong, Dayoung Kim, Chi Nam Seong, Sang Jip Nam, Kyung Min Lim

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18 Scopus citations


Bioactivity-guided isolation of a crude extract from a culture broth of Bacillus sp. has led to the isolation of (-)-4-hydroxysattabacin (1). The inhibitory effect of (-)-4-hydroxysattabacin (1) was investigated on melanogenesis in the murine melanoma cell line, B16F10, and human melanoma cell line, MNT-1, as well as a pigmented 3D-human skin model. (-)-4-Hydroxysattabacin treatment decreased melanin contents in a dose-dependent manner in α-melanocyte stimulating hormone (α-MSH)-stimulated B16F10 cells. Quantitative real time PCR (qRT-PCR) demonstrated that treatment with (-)-4-hydroxysattabacin down-regulated several melanogenic genes, including tyrosinase, tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2) while their enzymatic activities were unaffected. The anti-melanogenic effects of (-)-4-hydroxysattabacin were further demonstrated in a pigmented 3D human epidermal skin model, Melanoderm™, and manifested as whitening and regression of melanocyte activation in the tissue.

Original languageEnglish
Article number138
JournalMarine Drugs
Issue number5
StatePublished - May 2017

Bibliographical note

Funding Information:
The present study was supported by a grant of the Korean Health Technology R and D Project, Ministry of Health and Welfare, Republic of Korea (Grant No. HN15C0102) and by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT and Future Planning (NRF- 2014R1A1A1003492).

Publisher Copyright:
© 2017 by the authors. Licensee MDPI.


  • (-)-4-Hydroxysattabacin
  • Anti-pigmentary effect
  • Bacillus sp.
  • Marine-derived
  • Melanogenesis

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