Abstract
Bioactivity-guided isolation of a crude extract from a culture broth of Bacillus sp. has led to the isolation of (-)-4-hydroxysattabacin (1). The inhibitory effect of (-)-4-hydroxysattabacin (1) was investigated on melanogenesis in the murine melanoma cell line, B16F10, and human melanoma cell line, MNT-1, as well as a pigmented 3D-human skin model. (-)-4-Hydroxysattabacin treatment decreased melanin contents in a dose-dependent manner in α-melanocyte stimulating hormone (α-MSH)-stimulated B16F10 cells. Quantitative real time PCR (qRT-PCR) demonstrated that treatment with (-)-4-hydroxysattabacin down-regulated several melanogenic genes, including tyrosinase, tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2) while their enzymatic activities were unaffected. The anti-melanogenic effects of (-)-4-hydroxysattabacin were further demonstrated in a pigmented 3D human epidermal skin model, Melanoderm™, and manifested as whitening and regression of melanocyte activation in the tissue.
Original language | English |
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Article number | 138 |
Journal | Marine Drugs |
Volume | 15 |
Issue number | 5 |
DOIs | |
State | Published - May 2017 |
Keywords
- (-)-4-Hydroxysattabacin
- Anti-pigmentary effect
- Bacillus sp.
- Marine-derived
- Melanogenesis