Anti-inflammatory effects of silkworm hemolymph on lipopolysaccharide-stimulated macrophages

Mi Ra Chang; Woong Hee Lee; Won Jong Rhee; Tai Hyun Park; Eun Jeong Kim

doi:10.1007/s11814-013-0108-6

Anti-inflammatory effects of silkworm hemolymph on lipopolysaccharide-stimulated macrophages

Mi Ra Chang, Woong Hee Lee, Won Jong Rhee, Tai Hyun Park, Eun Jeong Kim

Department of Nutritional Science & Food Management

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Macrophages participate in several inflammatory pathologies such as sepsis and arthritis. We investigated the effect of silkworm hemolymph (SH) on the LPS-induced pro-inflammatory macrophages. SH inhibits LPS-induced nitric oxide (NO) production in RAW 264.7 cells and murine peritoneal macrophages. The decreased NO was reflected as a decreased amount of inducible nitric oxide synthase (iNOS) mRNA and protein. It was also found that SH inhibited pro-inflammatory cytokines, IL-1β, IL-6, and TNF-α production. To elucidate the mechanism by which SH inhibits NO production and iNOS expression, we investigated that SH suppressed IκB phosphorylation, which leads to the activation of NF-κB followed by degradation of IκB. This observation suggests that SH is a potential therapeutic modulator for inflammation-associated disorders.

Original language	English
Pages (from-to)	1784-1789
Number of pages	6
Journal	Korean Journal of Chemical Engineering
Volume	30
Issue number	9
DOIs	https://doi.org/10.1007/s11814-013-0108-6
State	Published - Sep 2013

Bibliographical note

Funding Information:
This work was supported by grants from the Korea Research Institute of Standards and Science (KRISS) (2007). T.H.P. acknowledges the support from the Pioneer Research Program and the Bio & Medical Technology Development Program, through the National Research Foundation of Korea (NRF) funded by the Korean government (MEST) (No. 20120001020 and 2012050100).

Keywords

Inducible Nitric Oxide Synthase (iNOS)
Macrophages
Nitric Oxide (NO)
Pro-inflammatory Cytokines
Silkworm Hemolymph

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title = "Anti-inflammatory effects of silkworm hemolymph on lipopolysaccharide-stimulated macrophages",

abstract = "Macrophages participate in several inflammatory pathologies such as sepsis and arthritis. We investigated the effect of silkworm hemolymph (SH) on the LPS-induced pro-inflammatory macrophages. SH inhibits LPS-induced nitric oxide (NO) production in RAW 264.7 cells and murine peritoneal macrophages. The decreased NO was reflected as a decreased amount of inducible nitric oxide synthase (iNOS) mRNA and protein. It was also found that SH inhibited pro-inflammatory cytokines, IL-1β, IL-6, and TNF-α production. To elucidate the mechanism by which SH inhibits NO production and iNOS expression, we investigated that SH suppressed IκB phosphorylation, which leads to the activation of NF-κB followed by degradation of IκB. This observation suggests that SH is a potential therapeutic modulator for inflammation-associated disorders.",

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author = "Chang, {Mi Ra} and Lee, {Woong Hee} and Rhee, {Won Jong} and Park, {Tai Hyun} and Kim, {Eun Jeong}",

note = "Funding Information: This work was supported by grants from the Korea Research Institute of Standards and Science (KRISS) (2007). T.H.P. acknowledges the support from the Pioneer Research Program and the Bio & Medical Technology Development Program, through the National Research Foundation of Korea (NRF) funded by the Korean government (MEST) (No. 20120001020 and 2012050100).",

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doi = "10.1007/s11814-013-0108-6",

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AU - Lee, Woong Hee

AU - Rhee, Won Jong

AU - Park, Tai Hyun

AU - Kim, Eun Jeong

N1 - Funding Information: This work was supported by grants from the Korea Research Institute of Standards and Science (KRISS) (2007). T.H.P. acknowledges the support from the Pioneer Research Program and the Bio & Medical Technology Development Program, through the National Research Foundation of Korea (NRF) funded by the Korean government (MEST) (No. 20120001020 and 2012050100).

PY - 2013/9

Y1 - 2013/9

N2 - Macrophages participate in several inflammatory pathologies such as sepsis and arthritis. We investigated the effect of silkworm hemolymph (SH) on the LPS-induced pro-inflammatory macrophages. SH inhibits LPS-induced nitric oxide (NO) production in RAW 264.7 cells and murine peritoneal macrophages. The decreased NO was reflected as a decreased amount of inducible nitric oxide synthase (iNOS) mRNA and protein. It was also found that SH inhibited pro-inflammatory cytokines, IL-1β, IL-6, and TNF-α production. To elucidate the mechanism by which SH inhibits NO production and iNOS expression, we investigated that SH suppressed IκB phosphorylation, which leads to the activation of NF-κB followed by degradation of IκB. This observation suggests that SH is a potential therapeutic modulator for inflammation-associated disorders.

AB - Macrophages participate in several inflammatory pathologies such as sepsis and arthritis. We investigated the effect of silkworm hemolymph (SH) on the LPS-induced pro-inflammatory macrophages. SH inhibits LPS-induced nitric oxide (NO) production in RAW 264.7 cells and murine peritoneal macrophages. The decreased NO was reflected as a decreased amount of inducible nitric oxide synthase (iNOS) mRNA and protein. It was also found that SH inhibited pro-inflammatory cytokines, IL-1β, IL-6, and TNF-α production. To elucidate the mechanism by which SH inhibits NO production and iNOS expression, we investigated that SH suppressed IκB phosphorylation, which leads to the activation of NF-κB followed by degradation of IκB. This observation suggests that SH is a potential therapeutic modulator for inflammation-associated disorders.

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KW - Nitric Oxide (NO)

KW - Pro-inflammatory Cytokines

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JO - Korean Journal of Chemical Engineering

JF - Korean Journal of Chemical Engineering

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ER -

Anti-inflammatory effects of silkworm hemolymph on lipopolysaccharide-stimulated macrophages

Abstract

Bibliographical note

Keywords

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