Anti-inflammatory effects of silkworm hemolymph on lipopolysaccharide-stimulated macrophages

Mi Ra Chang, Woong Hee Lee, Won Jong Rhee, Tai Hyun Park, Eun Jeong Kim

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Macrophages participate in several inflammatory pathologies such as sepsis and arthritis. We investigated the effect of silkworm hemolymph (SH) on the LPS-induced pro-inflammatory macrophages. SH inhibits LPS-induced nitric oxide (NO) production in RAW 264.7 cells and murine peritoneal macrophages. The decreased NO was reflected as a decreased amount of inducible nitric oxide synthase (iNOS) mRNA and protein. It was also found that SH inhibited pro-inflammatory cytokines, IL-1β, IL-6, and TNF-α production. To elucidate the mechanism by which SH inhibits NO production and iNOS expression, we investigated that SH suppressed IκB phosphorylation, which leads to the activation of NF-κB followed by degradation of IκB. This observation suggests that SH is a potential therapeutic modulator for inflammation-associated disorders.

Original languageEnglish
Pages (from-to)1784-1789
Number of pages6
JournalKorean Journal of Chemical Engineering
Issue number9
StatePublished - Sep 2013

Bibliographical note

Funding Information:
This work was supported by grants from the Korea Research Institute of Standards and Science (KRISS) (2007). T.H.P. acknowledges the support from the Pioneer Research Program and the Bio & Medical Technology Development Program, through the National Research Foundation of Korea (NRF) funded by the Korean government (MEST) (No. 20120001020 and 2012050100).


  • Inducible Nitric Oxide Synthase (iNOS)
  • Macrophages
  • Nitric Oxide (NO)
  • Pro-inflammatory Cytokines
  • Silkworm Hemolymph


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