TY - JOUR
T1 - Anti-inflammatory effects of Rubus coreanus Miquel through inhibition of NF-κB and MAP kinase
AU - Lee, Jung Eun
AU - Cho, Soo Muk
AU - Park, Eunkyo
AU - Lee, Seung Min
AU - Kim, Yuri
AU - Auh, Joong Hyuck
AU - Choi, Hyung Kyoon
AU - Lim, Sohee
AU - Lee, Sung Chul
AU - Kim, Jung Hyun
N1 - Publisher Copyright:
© 2014 The Korean Nutrition Society and the Korean Society of Community Nutrition.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - MATERIALS/METHODS: Unripe RCM ethanol extract (UE), unripe RCM water extract (UH), ripe RCM ethanol extract (RE), and ripe RCM water extract (RH) were prepared. Inflammatory response was induced with LPS treatment, and expression of pro-inflammatory mediators (iNOS, COX-2, TNF-α, IL-1β, and IL-6) and NO and PGE2 productions were assessed. To determine the anti-inflammatory mechanism of RCM, we measured NF-κB and MAPK activities.RESULTS: UE and UH treatment significantly reduced NF-κB activation and JNK and p38 phosphorylation and reduced transcriptional activities decreased iNOS, COX-2, and pro-inflammatory cytokines expressions, and NO and PGE2 productions. RE and RH treatments reduced IL-1β and IL-6 expressions through suppressions of JNK and p38 phosphorylation.CONCLUSIONS: In this study, we showed that RCM had anti-inflammatory effects by suppression of pro-inflammatory mediator expressions. Especially, unripe RCM showed strong anti-inflammatory effects through suppression of NF-κB and MAPK activation. These findings suggest that unripe RCM might be used as a potential functional material to reduce chronic inflammatory responses.BACKGROUND/OBJECTIVES: Rubus Coreanus Miquel (RCM), used as a traditional Korean medicine, reduces chronic inflammatory diseases such as cancer and rheumatoid arthritis. However, its mechanism has not been elucidated. In this study, we examine the anti-inflammatory effects of RCM and their possible mechanisms using RAW 264.7 cells.
AB - MATERIALS/METHODS: Unripe RCM ethanol extract (UE), unripe RCM water extract (UH), ripe RCM ethanol extract (RE), and ripe RCM water extract (RH) were prepared. Inflammatory response was induced with LPS treatment, and expression of pro-inflammatory mediators (iNOS, COX-2, TNF-α, IL-1β, and IL-6) and NO and PGE2 productions were assessed. To determine the anti-inflammatory mechanism of RCM, we measured NF-κB and MAPK activities.RESULTS: UE and UH treatment significantly reduced NF-κB activation and JNK and p38 phosphorylation and reduced transcriptional activities decreased iNOS, COX-2, and pro-inflammatory cytokines expressions, and NO and PGE2 productions. RE and RH treatments reduced IL-1β and IL-6 expressions through suppressions of JNK and p38 phosphorylation.CONCLUSIONS: In this study, we showed that RCM had anti-inflammatory effects by suppression of pro-inflammatory mediator expressions. Especially, unripe RCM showed strong anti-inflammatory effects through suppression of NF-κB and MAPK activation. These findings suggest that unripe RCM might be used as a potential functional material to reduce chronic inflammatory responses.BACKGROUND/OBJECTIVES: Rubus Coreanus Miquel (RCM), used as a traditional Korean medicine, reduces chronic inflammatory diseases such as cancer and rheumatoid arthritis. However, its mechanism has not been elucidated. In this study, we examine the anti-inflammatory effects of RCM and their possible mechanisms using RAW 264.7 cells.
KW - Cytokines
KW - Inflammation
KW - MAPK
KW - NF-κB
KW - Rubus coreanus Miquel
UR - http://www.scopus.com/inward/record.url?scp=84908071632&partnerID=8YFLogxK
U2 - 10.4162/nrp.2014.8.5.501
DO - 10.4162/nrp.2014.8.5.501
M3 - Article
AN - SCOPUS:84908071632
SN - 1976-1457
VL - 8
SP - 501
EP - 508
JO - Nutrition Research and Practice
JF - Nutrition Research and Practice
IS - 5
ER -