Anti-inflammatory effect of unripe fruit of Citrus grandis Osbeck in RAW264.7 and HaCaT cells

Hye Ja Lee; Gyeoung Jin Kang; Weon Jong Yoon; Hee Kyoung Kang; Young Suk Kim; So Mi Kim; Eun Sook Yoo

Anti-inflammatory effect of unripe fruit of Citrus grandis Osbeck in RAW264.7 and HaCaT cells

Hye Ja Lee, Gyeoung Jin Kang, Weon Jong Yoon, Hee Kyoung Kang, Young Suk Kim, So Mi Kim, Eun Sook Yoo

Department of Food Science & Biotechnology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

We investigated the anti-inflammatory activities of unripe fruit of Citrus grandis Osbeck growing at Jeju Island, through the evaluation of their inhibitory effect on the production of inflammatory markers (IL-6, iNOS, COX, TARC and MDC) in RAW264.7 murine macrophage cells and HaCaT human keratinocyte cells. Among the sequential solvent fractions obtained from crude extract, hexane and chloroform (CHCl₃) fractions showed potential inhibitory activity on the mRNA expressions of IL-6, iNOS and COX-2 at the concentration of 100 μg/ml in RAW264.7 cells. Also, EtOAc fraction showed inhibitory activity on the thymus and activation-regulated chemokine (TARC)/CCL17 and macrophage-derived chemokine (MDC)/CCL22 at the concentration of 50 μg/ml in HaCaT cells. These results suggest that the unripe fruit of C. grandis may have anti-inflammatory activity through the suppression of inflammatory markers (IL-6, iNOS, COX, TARC and MDC).

Original language	English
Pages (from-to)	74-80
Number of pages	7
Journal	Korean Journal of Pharmacognosy
Volume	37
Issue number	2
State	Published - Jun 2006

Keywords

Citrus grandis Osbeck
COX
IL-6
iNOS
MDC
TARC

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title = "Anti-inflammatory effect of unripe fruit of Citrus grandis Osbeck in RAW264.7 and HaCaT cells",

abstract = "We investigated the anti-inflammatory activities of unripe fruit of Citrus grandis Osbeck growing at Jeju Island, through the evaluation of their inhibitory effect on the production of inflammatory markers (IL-6, iNOS, COX, TARC and MDC) in RAW264.7 murine macrophage cells and HaCaT human keratinocyte cells. Among the sequential solvent fractions obtained from crude extract, hexane and chloroform (CHCl3) fractions showed potential inhibitory activity on the mRNA expressions of IL-6, iNOS and COX-2 at the concentration of 100 μg/ml in RAW264.7 cells. Also, EtOAc fraction showed inhibitory activity on the thymus and activation-regulated chemokine (TARC)/CCL17 and macrophage-derived chemokine (MDC)/CCL22 at the concentration of 50 μg/ml in HaCaT cells. These results suggest that the unripe fruit of C. grandis may have anti-inflammatory activity through the suppression of inflammatory markers (IL-6, iNOS, COX, TARC and MDC).",

keywords = "Citrus grandis Osbeck, COX, IL-6, iNOS, MDC, TARC",

author = "Lee, {Hye Ja} and Kang, {Gyeoung Jin} and Yoon, {Weon Jong} and Kang, {Hee Kyoung} and Kim, {Young Suk} and Kim, {So Mi} and Yoo, {Eun Sook}",

year = "2006",

month = jun,

language = "English",

volume = "37",

pages = "74--80",

journal = "Korean Journal of Pharmacognosy",

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T1 - Anti-inflammatory effect of unripe fruit of Citrus grandis Osbeck in RAW264.7 and HaCaT cells

AU - Lee, Hye Ja

AU - Kang, Gyeoung Jin

AU - Yoon, Weon Jong

AU - Kang, Hee Kyoung

AU - Kim, Young Suk

AU - Kim, So Mi

AU - Yoo, Eun Sook

PY - 2006/6

Y1 - 2006/6

N2 - We investigated the anti-inflammatory activities of unripe fruit of Citrus grandis Osbeck growing at Jeju Island, through the evaluation of their inhibitory effect on the production of inflammatory markers (IL-6, iNOS, COX, TARC and MDC) in RAW264.7 murine macrophage cells and HaCaT human keratinocyte cells. Among the sequential solvent fractions obtained from crude extract, hexane and chloroform (CHCl3) fractions showed potential inhibitory activity on the mRNA expressions of IL-6, iNOS and COX-2 at the concentration of 100 μg/ml in RAW264.7 cells. Also, EtOAc fraction showed inhibitory activity on the thymus and activation-regulated chemokine (TARC)/CCL17 and macrophage-derived chemokine (MDC)/CCL22 at the concentration of 50 μg/ml in HaCaT cells. These results suggest that the unripe fruit of C. grandis may have anti-inflammatory activity through the suppression of inflammatory markers (IL-6, iNOS, COX, TARC and MDC).

AB - We investigated the anti-inflammatory activities of unripe fruit of Citrus grandis Osbeck growing at Jeju Island, through the evaluation of their inhibitory effect on the production of inflammatory markers (IL-6, iNOS, COX, TARC and MDC) in RAW264.7 murine macrophage cells and HaCaT human keratinocyte cells. Among the sequential solvent fractions obtained from crude extract, hexane and chloroform (CHCl3) fractions showed potential inhibitory activity on the mRNA expressions of IL-6, iNOS and COX-2 at the concentration of 100 μg/ml in RAW264.7 cells. Also, EtOAc fraction showed inhibitory activity on the thymus and activation-regulated chemokine (TARC)/CCL17 and macrophage-derived chemokine (MDC)/CCL22 at the concentration of 50 μg/ml in HaCaT cells. These results suggest that the unripe fruit of C. grandis may have anti-inflammatory activity through the suppression of inflammatory markers (IL-6, iNOS, COX, TARC and MDC).

KW - Citrus grandis Osbeck

KW - COX

KW - IL-6

KW - iNOS

KW - MDC

KW - TARC

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M3 - Article

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SN - 0253-3073

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EP - 80

JO - Korean Journal of Pharmacognosy

JF - Korean Journal of Pharmacognosy

IS - 2

ER -

Anti-inflammatory effect of unripe fruit of Citrus grandis Osbeck in RAW264.7 and HaCaT cells

Abstract

Keywords

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