Anti-atherogenic effect of citrus flavonoids, naringin and naringenin, associated with hepatic ACAT and aortic VCAM-1 and MCP-1 in high cholesterol-fed rabbits

Chul Ho Lee, Tae Sook Jeong, Yang Kyu Choi, Byung Hwa Hyun, Goo Taeg Oh, Eun Hee Kim, Ju Ryoung Kim, Jang Il Han, Song Hae Bok

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210 Scopus citations

Abstract

The anti-atherogenic effects of the citrus flavonoids, naringin and naringenin, were evaluated in high cholesterol-fed rabbits. At 3 months of age, 30 male New Zealand White (NZW) rabbits were divided into three groups (n=10 per group). The rabbits were fed a 1% cholesterol diet alone (control group) or a diet supplemented with either 0.1% naringin or 0.05% naringenin for 8 weeks. The plasma lipoprotein levels, total cholesterol, triglyceride, and high-density lipoprotein showed no significant differences in the control and experimental groups. Hepatic acyl-CoA: cholesterol acyltransferase (ACAT) activity was slightly low in naringin (5.0%)- and naringenin (15.0%)-fed rabbits, compared to control group. The aortic fatty streak areas were significantly lower in both the naringin (19.2 ± 5.6%)- and naringenin (18.1 ± 6.5%)-supplemented groups than in the control group (60.4 ± 14.0%). The expression levels of vascular cell adhesion molecule-1 (VCAM-1) and monocyte chemotactic protein-1 (MCP-1), by semiquantitative RT-PCR analysis of the thoracic aorta, were significantly lower in the flavonoids supplemented groups than in the control group. These results suggest that the anti-atherogenic effect of the citrus flavonoids, naringin and naringenin, is involved with a decreased hepatic ACAT activity and with the downregulation of VCAM-1 and MCP-1 gene expression.

Original languageEnglish
Pages (from-to)681-688
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume284
Issue number3
DOIs
StatePublished - 2001

Keywords

  • ACAT
  • Fatty streak
  • MCP-1
  • Naringenin
  • Naringin
  • VCAM-1

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