Anithiactin D, a Phenylthiazole Natural Product from Mudflat-Derived Streptomyces sp., Suppresses Motility of Cancer Cells

Sultan Pulat; Inho Yang; Jihye Lee; Sunghoon Hwang; Rui Zhou; Chathurika D.B. Gamage; Mücahit Varlı; İsa Taş; Yi Yang; So Yeon Park; Ahreum Hong; Jeong Hyeon Kim; Dong Chan Oh; Hangun Kim; Sang Jip Nam; Heonjoong Kang

doi:10.3390/md22020088

Anithiactin D, a Phenylthiazole Natural Product from Mudflat-Derived Streptomyces sp., Suppresses Motility of Cancer Cells

Sultan Pulat, Inho Yang, Jihye Lee, Sunghoon Hwang, Rui Zhou, Chathurika D.B. Gamage, Mücahit Varlı, İsa Taş, Yi Yang, So Yeon Park, Ahreum Hong, Jeong Hyeon Kim, Dong Chan Oh, Hangun Kim, Sang Jip Nam, Heonjoong Kang

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Anithiactin D (1), a 2-phenylthiazole class of natural products, was isolated from marine mudflat-derived actinomycetes Streptomyces sp. 10A085. The chemical structure of 1 was elucidated based on the interpretation of NMR and MS data. The absolute configuration of 1 was determined by comparing the experimental and calculated electronic circular dichroism (ECD) spectral data. Anithiactin D (1) significantly decreased cancer cell migration and invasion activities at a concentration of 5 μM via downregulation of the epithelial-to-mesenchymal transition (EMT) markers in A549, AGS, and Caco-2 cell lines. Moreover, 1 inhibited the activity of Rho GTPases, including Rac1 and RhoA in the A549 cell line, suppressed RhoA in AGS and Caco-2 cell lines, and decreased the mRNA expression levels of some matrix metalloproteinases (MMPs) in AGS and Caco-2 cell lines. Thus 1, which is a new entity of the 2-phenylthiazole class of natural products with a unique aniline-indole fused moiety, is a potent inhibitor of the motility of cancer cells.

Original language	English
Article number	88
Journal	Marine Drugs
Volume	22
Issue number	2
DOIs	https://doi.org/10.3390/md22020088
State	Published - Feb 2024

Bibliographical note

Publisher Copyright:
© 2024 by the authors.

Keywords

EMT
MMP
Rho GTPases
colorectal cancer
gastric cancer
lung cancer
marine natural products

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/md22020088

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Pulat, S., Yang, I., Lee, J., Hwang, S., Zhou, R., Gamage, C. D. B., Varlı, M., Taş, İ., Yang, Y., Park, S. Y., Hong, A., Kim, J. H., Oh, D. C., Kim, H., Nam, S. J., & Kang, H. (2024). Anithiactin D, a Phenylthiazole Natural Product from Mudflat-Derived Streptomyces sp., Suppresses Motility of Cancer Cells. Marine Drugs, 22(2), Article 88. https://doi.org/10.3390/md22020088

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title = "Anithiactin D, a Phenylthiazole Natural Product from Mudflat-Derived Streptomyces sp., Suppresses Motility of Cancer Cells",

abstract = "Anithiactin D (1), a 2-phenylthiazole class of natural products, was isolated from marine mudflat-derived actinomycetes Streptomyces sp. 10A085. The chemical structure of 1 was elucidated based on the interpretation of NMR and MS data. The absolute configuration of 1 was determined by comparing the experimental and calculated electronic circular dichroism (ECD) spectral data. Anithiactin D (1) significantly decreased cancer cell migration and invasion activities at a concentration of 5 μM via downregulation of the epithelial-to-mesenchymal transition (EMT) markers in A549, AGS, and Caco-2 cell lines. Moreover, 1 inhibited the activity of Rho GTPases, including Rac1 and RhoA in the A549 cell line, suppressed RhoA in AGS and Caco-2 cell lines, and decreased the mRNA expression levels of some matrix metalloproteinases (MMPs) in AGS and Caco-2 cell lines. Thus 1, which is a new entity of the 2-phenylthiazole class of natural products with a unique aniline-indole fused moiety, is a potent inhibitor of the motility of cancer cells.",

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AU - Pulat, Sultan

AU - Yang, Inho

AU - Lee, Jihye

AU - Hwang, Sunghoon

AU - Zhou, Rui

AU - Gamage, Chathurika D.B.

AU - Varlı, Mücahit

AU - Taş, İsa

AU - Yang, Yi

AU - Park, So Yeon

AU - Hong, Ahreum

AU - Kim, Jeong Hyeon

AU - Oh, Dong Chan

AU - Kim, Hangun

AU - Nam, Sang Jip

AU - Kang, Heonjoong

PY - 2024/2

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N2 - Anithiactin D (1), a 2-phenylthiazole class of natural products, was isolated from marine mudflat-derived actinomycetes Streptomyces sp. 10A085. The chemical structure of 1 was elucidated based on the interpretation of NMR and MS data. The absolute configuration of 1 was determined by comparing the experimental and calculated electronic circular dichroism (ECD) spectral data. Anithiactin D (1) significantly decreased cancer cell migration and invasion activities at a concentration of 5 μM via downregulation of the epithelial-to-mesenchymal transition (EMT) markers in A549, AGS, and Caco-2 cell lines. Moreover, 1 inhibited the activity of Rho GTPases, including Rac1 and RhoA in the A549 cell line, suppressed RhoA in AGS and Caco-2 cell lines, and decreased the mRNA expression levels of some matrix metalloproteinases (MMPs) in AGS and Caco-2 cell lines. Thus 1, which is a new entity of the 2-phenylthiazole class of natural products with a unique aniline-indole fused moiety, is a potent inhibitor of the motility of cancer cells.

AB - Anithiactin D (1), a 2-phenylthiazole class of natural products, was isolated from marine mudflat-derived actinomycetes Streptomyces sp. 10A085. The chemical structure of 1 was elucidated based on the interpretation of NMR and MS data. The absolute configuration of 1 was determined by comparing the experimental and calculated electronic circular dichroism (ECD) spectral data. Anithiactin D (1) significantly decreased cancer cell migration and invasion activities at a concentration of 5 μM via downregulation of the epithelial-to-mesenchymal transition (EMT) markers in A549, AGS, and Caco-2 cell lines. Moreover, 1 inhibited the activity of Rho GTPases, including Rac1 and RhoA in the A549 cell line, suppressed RhoA in AGS and Caco-2 cell lines, and decreased the mRNA expression levels of some matrix metalloproteinases (MMPs) in AGS and Caco-2 cell lines. Thus 1, which is a new entity of the 2-phenylthiazole class of natural products with a unique aniline-indole fused moiety, is a potent inhibitor of the motility of cancer cells.

KW - EMT

KW - MMP

KW - Rho GTPases

KW - colorectal cancer

KW - gastric cancer

KW - lung cancer

KW - marine natural products

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SN - 1660-3397

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JO - Marine Drugs

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ER -

Anithiactin D, a Phenylthiazole Natural Product from Mudflat-Derived Streptomyces sp., Suppresses Motility of Cancer Cells

Abstract

Bibliographical note

Keywords

UN SDGs

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