Analysis of two-stage continuous operation of Escherichia coli containing bacteriophage λ vector

Hoon Park Seong Hoon Park, Hyun Park Tai Hyun Park

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7 Scopus citations


Bacteriophage λ containing a cloned-gene is stably maintained in Escherichia coli in the lysogenic state while it is replicated and it overproduces a recombinant protein product in the lytic state. The host cell is eventually lysed in the lytic state. The kinetics of cell lysis and production induction were studied and are reported in this article through model equations. In two-stage continuous operation, the first tank is maintained in the lysogenic state for cell growth and cloned-gene stability while the second tank is in the lytic state for the overproduction of cloned-gene product. Individual cells in the second tank have different extent of the induction for product formation, since each has a different residence time. The different residence time for individual cells was taken into account using a population model. The numerical results show good agreement with the experimental data for the prediction of dilution rate in the second tank which gives the maximum product concentration.

Original languageEnglish
Pages (from-to)557-563
Number of pages7
JournalBioprocess Engineering
Issue number6
StatePublished - 2000

Bibliographical note

Funding Information:
Seong Hoon Park, Tai Hyun Park (&) School of Chemical Engineering, Seoul National University, Kwanak-Gu Shilim-Dong San 56-1, Seoul 151-742, Korea The authors wish to acknowledge the financial support of the Korea Science & Engineering Αoundation through Nano BioElectronic & System Center. This work was supported in parts by the Brain Korea 21 proΒect.


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