A new aggregation-induced emission and excited-state intramolecular proton transfer based fluorescent probe, containing a salicylaldazine moiety as a platform, displayed an excellent light-up ratio and a large Stokes shift for the detection of biothiols (cysteine, homocysteine, and glutathione). The salicylaldazine based fluorescent probe showed high selectivity, and sensitivity for biothiols. With the aid of different reactivities, a kinetically resolved method was successfully applied to distinguish different biothiols both in solution and cells.
Bibliographical noteFunding Information:
The authors acknowledge a grant from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (No. 2012R1A3A2048814).
© The Royal Society of Chemistry 2016.