@article{0d7eddb6f7eb48b7b3c7f2090d5a8d26,
title = "Alleviation of memory deficit by bergenin via the regulation of reelin and Nrf-2/NF-κB pathway in transgenic mouse model",
abstract = "The present study aims to determine the neuroprotective effect of Bergenin against spatial memory deficit associated with neurodegeneration. Preliminarily, the protective effect of Bergenin was observed against H2 O2-induced oxidative stress in HT-22 and PC-12 cells. Further studies were performed in 5xFAD Tg mouse model by administering Bergenin (1, 30 and 60 mg/kg; orally), whereas Bergenin (60 mg/kg) significantly attenuated the memory deficit observed in the Y-maze and Morris water maze (MWM) test. Fourier transform-infrared (FT-IR) spectroscopy displayed restoration of lipids, proteins and their derivatives compared to the 5xFAD Tg mice group. The differential scanning calorimeter (DSC) suggested an absence of amyloid beta (Aβ) aggregation in Bergenin-treated mice. The immunohistochemistry (IHC) analysis suggested the neuroprotective effect of Bergenin by increasing Reelin signaling (Reelin/Dab-1) and attenuated Aβ (1–42) aggregation in hippocampal regions of mouse brains. Furthermore, IHC and western blot results suggested antioxidant (Keap-1/Nrf-2/HO-1), anti-inflammatory (TLR-4/NF-kB) and anti-apoptotic (Bcl-2/Bax/Caspase-3) effect of Bergenin. Moreover, a decrease in Annexin V/PI-stained hippocampal cells suggested its effect against neurodegeneration. The histopathological changes were reversed significantly by Bergenin. In addition, a remarkable increase in antioxidant level with suppression of pro-inflammatory cytokines, oxidative stress and nitric oxide production were observed in specific regions of the mouse brains.",
keywords = "Alzheimer{\textquoteright}s disease, Bergenin, Memory loss, Neurode-generation, Oxidative stress, Reelin signaling",
author = "Bushra Shal and Adnan Khan and Khan, {Ashraf Ullah} and Rahim Ullah and Gowhar Ali and Islam, {Salman Ul} and Haq, {Ihsan Ul} and Hussain Ali and Seo, {Eun Kyoung} and Salman Khan",
note = "Funding Information: curation, resources, and validation. S.K.: conceptualization, visualization, validation, data curation, Funding: The National Research Foundation of Korea (NRF) supported this work; the Korean govetrontmheenptu (bMliSshITe)d(Nveor.sNioRnFo-2f0t2h1eRm1Aan2uCs1c0r0ip33t.50) funded grant. The authors are also grateful to the Higher Education Commission (HEC), Pakistan, for supporting under the Indigenous Fellowship Funding: The National Research Foundation of Korea (NRF) supported this work; the Korean (No.17-5(Ph-II)/2MD4-051/HEC/IS/2017). government (MSIT) (No.NRF-2021R1A2C1003350) funded grant. The authors are also grateful to the IHnsitgihtuetrioEndaulcRateivoinewC oBmomarids sSiotante(HmEenCt):, TPhaek sistutadny, fworass ucponpdourtcitnegd uanccdoerrdtihneg Itnod tihgeeNnoautisonFaell lIonwstsih-ip t(uNteo o.1f7 H-5e(Palhth-I I(N)/I2HM) DG4u-i0d5e1l/inHesE fCo/r ItSh/e2 C01a7r)e. and Use of Laboratory Animals and was approved by the Bioethical Committee of University of Peshawar (UOP) with approval No. 12/EC-17/Pharm. Institutional Review Board Statement: The study was conducted according to the National Institute of Health (NIH) Guidelines for the Care and Use of Laboratory Animals and was approved by the Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2021",
month = jun,
day = "2",
doi = "10.3390/ijms22126603",
language = "English",
volume = "22",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "12",
}