Alba from Thermoplasma volcanium belongs to α-NAT's: An insight into the structural aspects of Tv Alba and its acetylation by Tv Ard1

Chao Ma, Chinar Pathak, Sang Jae Lee, Ki Young Lee, Sun Bok Jang, Minjoo Nam, Hookang Im, Hye Jin Yoon, Bong Jin Lee

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The Alba superfamily proteins have been regarded as a conserved group of proteins in archaea and eukarya, which have shown to be important in nucleic acid binding, chromatic organization and gene regulation. These proteins often belong to the N-acetyltransferase (NAT) category (Nα-acetyltransferases or Nε-acetyltransferases) and undergo post-translational modifications. Here, we report the crystal structure of Alba from Thermoplasma volcanium (Tv Alba) at 2.4 Å resolution. The acetylation of Tv Alba was monitored and the N-terminal of Tv Alba has been shown to interact with acetyl coenzyme A (Ac-CoA). The chemical shift perturbation experiments of Tv Alba were performed in the presence of Ac-CoA and/or Tv Ard1, another T. volcanium protein that treats Tv Alba as a substrate. To examine the DNA binding capabilities of Tv Alba alone and in the presence of Ac-CoA and/or Tv Ard1, EMSA experiments were carried out. It is shown that although Tv Alba binds to Ac-CoA, the acetylation of Tv Alba is not related with its binding to dsDNA, and the involvement of the N-terminus in Ac-CoA binding demonstrates that Tv Alba belongs to the Nα-acetyltransferase family.

Original languageEnglish
Pages (from-to)90-100
Number of pages11
JournalArchives of Biochemistry and Biophysics
Volume590
DOIs
StatePublished - 15 Jan 2016

Bibliographical note

Funding Information:
The authors thank the staff at Pohang Accelerator Laboratory (PAL), Korea for assisting during X-ray data collection experiments. This work was supported by the National Research Foundation of Korea (NRF) grant [ 20070056817 , 2012R1A2A1A01003569 , 2014K1A3A1A19067618 and 2014R1A1A3A04050250 ] funded by the Korean Government; and the 2015 BK21 Plus Project for Medicine, Dentistry and Pharmacy.

Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.

Keywords

  • Alba
  • Ard1
  • Crystallography
  • N-terminal acetyltransferase
  • NMR
  • Thermoplasma volcanium

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