In biological olfactory systems, olfactory receptors (ORs) can recognize and discriminate between thousands of volatile organic compounds with very high sensitivity and specificity. The superior properties of ORs have led to the development of OR-based biosensors that have shown promising potential in many applications over the past two decades. In particular, newly designed technologies in gene synthesis, protein expression, solubilization, purification, and membrane mimetics for membrane proteins have greatly opened up the previously inaccessible industrial potential of ORs. In this review, gene design, expression and solubilization strategies, and purification and reconstitution methods available for modern industrial applications are examined, with a focus on ORs. The limitations of current OR production technology are also estimated, and future directions for further progress are suggested.
Bibliographical noteFunding Information:
This study was supported by a grant (NRF‐2018R1A2B3004498) of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (MSIT), Republic of Korea; and the Technology Innovation Program (20020801, Construction of international standardization basis for the biodigital biosense) funded by the Ministry of Trade, Industry & Energy (MOTIE), Republic of Korea.
© 2023 Wiley-VCH GmbH.
- bioelectronic noses
- membrane mimetics
- membrane protein production
- olfactory receptors
- protein expression