Adaptation of Methanosarcina barkeri 227 as acetate scavenger for succinate fermentation by Actinobacillus succinogenes

Su Nyoung Kim, Young Bin Cho, Ji Won Park, Ok Bin Kim

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Acetate is the main by-product from microbial succinate production. In this study, we performed acetate removal by Methanosarcina barkeri 227 for succinate fermentation by Actinobacillus succinogenes 130Z. The acetoclastic methanogen M. barkeri requires similar environmental factors to A. succinogenes, and the conditions required for co-cultivation were optimized in this study: gas used for anaerobicization, strain adaptation, medium composition, pH adjustment, and inoculation time points. M. barkeri 227 was adapted to acetate for 150 days, which accelerated the acetate consumption to 9-fold (from 190 to 1726 mmol gDW−1 day−1). In the acetate-adapted strain, there was a noticeable increase in transcription of genes required for acetoclastic pathway—satP (acetate transporter), ackA (acetate kinase), cdhA (carbon monoxide dehydrogenase/acetyl-CoA synthase complex), and mtrH (methyl-H4STP:CoM methyltransferase), which was not induced before the adaptation process. The activities of two energy-consuming steps in the pathway—acetate uptake and acetate kinase—increased about 3-fold. This acetate-adapted M. barkeri could be successfully applied to succinate fermentation culture of A. succinogenes, but only after pH adjustment following completion of fermentation. This study suggests the utility of M. barkeri as an acetate scavenger during fermentation for further steps towards genetic and process engineering.

Original languageEnglish
Pages (from-to)4483-4492
Number of pages10
JournalApplied Microbiology and Biotechnology
Issue number10
StatePublished - 1 May 2020

Bibliographical note

Funding Information:
This study was supported by the National Research Foundation grant NRF-2015R1A4A1041997 and NRF-2019R1A2C1008066 funded by the government of Korea. Acknowledgments

Publisher Copyright:
© 2020, Springer-Verlag GmbH Germany, part of Springer Nature.


  • Acetate scavenger
  • Actinobacillus succinogenes
  • Bioculture
  • Methanosarcina barkeri
  • Succinate fermentation


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