Activity-dependent neuronal cell migration induced by electrical stimulation

Se Hoon Jeong, Sang Beom Jun, Jong Keun Song, Sung June Kim

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Recently, we found that electrical stimulation can induce neuronal migration in neural networks cultured for more than 3 weeks on microelectrode arrays. Immunocytochemistry data showed that the aggregation of neurons was related to the emergence of astrocytes in culture. In this study, when neurons were cocultured with astrocytes, electrical stimulation could induce the migration of neuronal cell bodies after only 1 week in culture, while the same stimulation paradigm caused neural necrosis in neuron-only cultures. In addition, the stimulation-induced migration was inhibited by blocking action potentials in neural networks using the voltage-gated sodium channel blocker, tetrodotoxin. Immunocytochemistry was performed to monitor precisely the neuronal migration and count the number of neurons. These results indicate that neuronal migration of cell bodies is dependent on neuronal activity evoked by electrical stimulation and can be enhanced by coculturing with astrocytes. We believe this method can be employed as a means for modifying neural networks and improving the interface between electrodes and neurons.

Original languageEnglish
Pages (from-to)93-99
Number of pages7
JournalMedical and Biological Engineering and Computing
Issue number1
StatePublished - 2009

Bibliographical note

Funding Information:
Acknowledgments This work was supported by the Korea Science and Engineering Foundation (KOSEF) through the Nano Bioelectronics and Systems Research Center [Grant No. R11-2000-075-00002-0 for domestic research and R11-330 2000-075-01001-0 for international collaboration (June)].


  • Astrocyte
  • Electrical stimulation
  • Microelectrode array
  • Neuronal activity
  • Neuronal migration


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