Activity-Based NIR Enzyme Fluorescent Probes for the Diagnosis of Tumors and Image-Guided Surgery

Haidong Li, Dayeh Kim, Qichao Yao, Haoying Ge, Jeewon Chung, Jiangli Fan, Jingyun Wang, Xiaojun Peng, Juyoung Yoon

Research output: Contribution to journalReview articlepeer-review

229 Scopus citations


Near-infrared (NIR) activatable fluorescent probes have been considered to be the effective edge tools for the investigation of cell biology and disease diagnosis because of their outstanding advantages. Related genes involved in tumor genesis and progression regulate the overexpression of certain enzymes. Owing to the distinctive characteristics of quick reaction time and favorable pharmacokinetics, enzyme-reactive NIR optical probes have shown great potential in the diagnosis of tumorigenesis and in image-guided intraoperative surgeries with high signal-to-noise ratios. In this review, we mainly summarize the latest advancements in enzyme-reactive NIR fluorescent probes from design strategy to biomedical application. Specifically, some challenges and prospects in this field are presented at the end of the review, which will be beneficial to innovatively construct new multifunctional fluorescent probes and actively promote their clinical transformation in the future.

Original languageEnglish
Pages (from-to)17268-17289
Number of pages22
JournalAngewandte Chemie - International Edition
Issue number32
StatePublished - 2 Aug 2021

Bibliographical note

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© 2020 Wiley-VCH GmbH


  • enzyme activity
  • image-guided surgery
  • near-infrared activation
  • tumors


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