TY - JOUR
T1 - Activities of different cysteine proteases of Paragonimus westermani in cleaving human IgG.
AU - Chung, Y. B.
AU - Yang, H. J.
AU - Kang, S. Y.
AU - Kong, Y.
AU - Cho, S. Y.
PY - 1997/6
Y1 - 1997/6
N2 - Cleaving host immunoglobulins is a well known mechanism of evading host immune reactions exploited by helminth parasites. Secreted cysteine proteases of helminth are a part of enzymes cleaving host IgG. Paragonimus westermani produces at least six different species of the cysteine protease in its developmental stages. This study was undertaken to evaluate comparatively the activities against human IgG by the different enzymes. When the metacercariae, which secrete 27 and 28 kDa cysteine proteases, were incubated in human IgG solution, IgG was degraded at its hinge region. Further incubation resulted complete hydrolysis. From 4-week and 7-week old juveniles and 16-week old adults of P. westermani, five different enzymes at 15, 17, 27, 28 and 53 kDa have been purified, if the enzyme with the same molecular mass is regarded to be identical. In cleaving human IgG, each cysteine protease exhibited decreasing activities with age.
AB - Cleaving host immunoglobulins is a well known mechanism of evading host immune reactions exploited by helminth parasites. Secreted cysteine proteases of helminth are a part of enzymes cleaving host IgG. Paragonimus westermani produces at least six different species of the cysteine protease in its developmental stages. This study was undertaken to evaluate comparatively the activities against human IgG by the different enzymes. When the metacercariae, which secrete 27 and 28 kDa cysteine proteases, were incubated in human IgG solution, IgG was degraded at its hinge region. Further incubation resulted complete hydrolysis. From 4-week and 7-week old juveniles and 16-week old adults of P. westermani, five different enzymes at 15, 17, 27, 28 and 53 kDa have been purified, if the enzyme with the same molecular mass is regarded to be identical. In cleaving human IgG, each cysteine protease exhibited decreasing activities with age.
UR - http://www.scopus.com/inward/record.url?scp=0031156658&partnerID=8YFLogxK
U2 - 10.3347/kjp.1997.35.2.139
DO - 10.3347/kjp.1997.35.2.139
M3 - Article
C2 - 9241989
AN - SCOPUS:0031156658
SN - 0023-4001
VL - 35
SP - 139
EP - 142
JO - The Korean journal of parasitology
JF - The Korean journal of parasitology
IS - 2
ER -