Activation of microglia in kainic acid induced rat retinal apoptosis

Dong Hoon Shin, Hwa Young Lee, Hye Won Lee, Kyung Hoon Lee, Hyoung Soo Lim, Gye Sun Jeon, Sa Sun Cho, Douk Ho Hwang

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


We applied a variety of methods to follow the course of kainic acid (KA) induced retinal apoptosis, especially with regard to the spatial and temporal aspects. At 24 h after KA injection, a massive cell increase, which showed terminal transferase-mediated dUTP nick-end-labeling technique positive signals, was observed in all of the retinal layers, with the exception of the outer nuclear and photoreceptor layers. Electron microscopy further confirmed that these cells might be apoptotic body ingesting phagocytes, whose function seemed to correlate with bcl-2 mRNA up-regulation. When histochemical studies were performed to determine the cellular identity of the phagocytes, the microglia were thought to be the one and only type of phagocytes involved in the KA-induced retinal apoptosis. In conclusion, we demonstrated that after KA injection, microglia were the only phagocytes to participate in clearing apoptotic debris from the inner retinal layers, and that their function might correlate with the change in expression of the bcl-2 gene family. (C) 2000 Published by Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)159-162
Number of pages4
JournalNeuroscience Letters
Issue number3
StatePublished - 13 Oct 2000

Bibliographical note

Funding Information:
This study was supported by a grant (1999) from the Education and Research Foundation, Seoul National University College of Medicine.


  • Apoptosis
  • Immunohistochemistry
  • In situ hybridization
  • Kainic acid
  • Microglia
  • Rat
  • Retina
  • Terminal transferase-mediated dUTP nick-end-labeling staining


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