MRI and NIRF multimodal imaging probe was fabricated and investigated. Glycol chitosan coated SPION was decorated by MMP-2 substrate conjugating BHQ-3 and Cy5.5 dye at each end (BCMG-SPION). NIRF signals were recovered in the presence of MMP-2 at a sub-nanomolar level. BCMG-SPION produced the maximum NIRF intensity at 48 h post injection in vivo. Evaluation of the BCMG-SPION in the tumor tissue by MRI showed that relative contrast enhancement was 34% higher than normal muscles. NIRF images of excised organs showed the highest NIRF signal in tumor and the intensity was at least 8 times higher than other organs.
Bibliographical noteFunding Information:
This research was supported by the Basic Science Research Program through a National Research Foundation of Korea grant funded by the Korean Government (MEST) ( NRF-2017R1A5A1070259 ), National Research Foundation of Korea grant funded by the Korean Government (MSIT) ( NRF-2019R1A2C2008249 ) and the Global Research Lab (GRL) Program through the National Research Foundation of Korea grant funded by the Ministry of Science, ICT, and Future Planning ( NRF-2015K1A1A2072365 ). The Institute of Engineering Research at Seoul National University provided research facilities for this work.
© 2019 The Korean Society of Industrial and Engineering Chemistry
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