Accelerated Development of Hippocampal Neurons and Limited Adhesion of Astrocytes on Negatively Charged Surfaces

Mi Hee Kim, Ji Hun Park, Sunghoon Joo, Daewha Hong, Matthew Park, Ji Yu Choi, Hye Won Moon, Yang Gyun Kim, Kyungtae Kang, Insung S. Choi

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

This work examines the development of primary neurons and astrocytes on thoroughly controlled functional groups. Negatively charged surfaces presenting carboxylate (COO-) or sulfonate (SO3-) groups prove beneficial to neuronal behavior, in spite of their supposed repulsive electrostatic interactions with cellular membranes. The adhesion and survival of primary hippocampal neurons on negatively charged surfaces are comparable to or slightly better than those on positively charged (poly-d-lysine-coated) surfaces, and neuritogenesis and neurite outgrowth are accelerated on COO- and SO3- surfaces. Moreover, such favorable influences of the negatively charged surfaces are only seen in neurons but not for astrocytes. Our results indicate that the in vitro developmental behavior of primary hippocampal neurons is sophisticatedly modulated by angstrom-sized differences in chemical structure or the charge density of the surface. We believe that this work provides new implications for understanding neuron-material interfaces as well as for establishing new ways to fabricate neuro-active surfaces.

Original languageEnglish
Pages (from-to)1767-1774
Number of pages8
JournalLangmuir
Volume34
Issue number4
DOIs
StatePublished - 30 Jan 2018

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