Absence of DNA damage after 60-Hz electromagnetic field exposure combined with ionizing radiation, hydrogen peroxide, or c-Myc overexpression

Yeung Bae Jin; Seo Hyun Choi; Jae Seon Lee; Jae Kyung Kim; Ju Woon Lee; Seung Cheol Hong; Sung Ho Myung; Yun Sil Lee

doi:10.1007/s00411-013-0506-5

Absence of DNA damage after 60-Hz electromagnetic field exposure combined with ionizing radiation, hydrogen peroxide, or c-Myc overexpression

Yeung Bae Jin, Seo Hyun Choi, Jae Seon Lee, Jae Kyung Kim, Ju Woon Lee, Seung Cheol Hong, Sung Ho Myung, Yun Sil Lee

Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The principal objective of this study was to assess the DNA damage in a normal cell line system after exposure to 60 Hz of extremely low frequency magnetic field (ELF-MF) and particularly in combination with various external factors, via comet assays. NIH3T3 mouse fibroblast cells, WI-38 human lung fibroblast cells, L132 human lung epithelial cells, and MCF10A human mammary gland epithelial cells were exposed for 4 or 16 h to a 60-Hz, 1 mT uniform magnetic field in the presence or absence of ionizing radiation (IR, 1 Gy), H₂O₂ (50 μM), or c-Myc oncogenic activation. The results obtained showed no significant differences between the cells exposed to ELF-MF alone and the unexposed cells. Moreover, no synergistic or additive effects were observed after 4 or 16 h of pre-exposure to 1 mT ELF-MF or simultaneous exposure to ELF-MF combined with IR, H₂O₂, or c-Myc activation.

Original language	English
Pages (from-to)	93-101
Number of pages	9
Journal	Radiation and Environmental Biophysics
Volume	53
Issue number	1
DOIs	https://doi.org/10.1007/s00411-013-0506-5
State	Published - Mar 2014

Bibliographical note

Funding Information:
Acknowledgments This work was supported by a Power Generation & Electricity Delivery of the Korea Institute of Energy Technology, Evaluation, and Planning (KITEP) grant (No. 2009101030003E) funded by the Korean Ministry of Trade, Industry & Energy and was also supported by the Ewha Global Top 5 Grant 2012 of Ewha Womans University.

Keywords

Combined treatment
Comet olive tail moment for DNA damage
Extremely low frequency

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title = "Absence of DNA damage after 60-Hz electromagnetic field exposure combined with ionizing radiation, hydrogen peroxide, or c-Myc overexpression",

abstract = "The principal objective of this study was to assess the DNA damage in a normal cell line system after exposure to 60 Hz of extremely low frequency magnetic field (ELF-MF) and particularly in combination with various external factors, via comet assays. NIH3T3 mouse fibroblast cells, WI-38 human lung fibroblast cells, L132 human lung epithelial cells, and MCF10A human mammary gland epithelial cells were exposed for 4 or 16 h to a 60-Hz, 1 mT uniform magnetic field in the presence or absence of ionizing radiation (IR, 1 Gy), H2O2 (50 μM), or c-Myc oncogenic activation. The results obtained showed no significant differences between the cells exposed to ELF-MF alone and the unexposed cells. Moreover, no synergistic or additive effects were observed after 4 or 16 h of pre-exposure to 1 mT ELF-MF or simultaneous exposure to ELF-MF combined with IR, H2O2, or c-Myc activation.",

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AU - Hong, Seung Cheol

AU - Myung, Sung Ho

AU - Lee, Yun Sil

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Absence of DNA damage after 60-Hz electromagnetic field exposure combined with ionizing radiation, hydrogen peroxide, or c-Myc overexpression

Abstract

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Keywords

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