Absence of DNA damage after 60-Hz electromagnetic field exposure combined with ionizing radiation, hydrogen peroxide, or c-Myc overexpression

Yeung Bae Jin; Seo Hyun Choi; Jae Seon Lee; Jae Kyung Kim; Ju Woon Lee; Seung Cheol Hong; Sung Ho Myung; Yun Sil Lee

doi:10.1007/s00411-013-0506-5

Absence of DNA damage after 60-Hz electromagnetic field exposure combined with ionizing radiation, hydrogen peroxide, or c-Myc overexpression

Yeung Bae Jin, Seo Hyun Choi, Jae Seon Lee, Jae Kyung Kim, Ju Woon Lee, Seung Cheol Hong, Sung Ho Myung, Yun Sil Lee

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The principal objective of this study was to assess the DNA damage in a normal cell line system after exposure to 60 Hz of extremely low frequency magnetic field (ELF-MF) and particularly in combination with various external factors, via comet assays. NIH3T3 mouse fibroblast cells, WI-38 human lung fibroblast cells, L132 human lung epithelial cells, and MCF10A human mammary gland epithelial cells were exposed for 4 or 16 h to a 60-Hz, 1 mT uniform magnetic field in the presence or absence of ionizing radiation (IR, 1 Gy), H₂O₂ (50 μM), or c-Myc oncogenic activation. The results obtained showed no significant differences between the cells exposed to ELF-MF alone and the unexposed cells. Moreover, no synergistic or additive effects were observed after 4 or 16 h of pre-exposure to 1 mT ELF-MF or simultaneous exposure to ELF-MF combined with IR, H₂O₂, or c-Myc activation.

Original language	English
Pages (from-to)	93-101
Number of pages	9
Journal	Radiation and Environmental Biophysics
Volume	53
Issue number	1
DOIs	https://doi.org/10.1007/s00411-013-0506-5
State	Published - Mar 2014

Bibliographical note

Funding Information:
Acknowledgments This work was supported by a Power Generation & Electricity Delivery of the Korea Institute of Energy Technology, Evaluation, and Planning (KITEP) grant (No. 2009101030003E) funded by the Korean Ministry of Trade, Industry & Energy and was also supported by the Ewha Global Top 5 Grant 2012 of Ewha Womans University.

Keywords

Combined treatment
Comet olive tail moment for DNA damage
Extremely low frequency

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title = "Absence of DNA damage after 60-Hz electromagnetic field exposure combined with ionizing radiation, hydrogen peroxide, or c-Myc overexpression",

abstract = "The principal objective of this study was to assess the DNA damage in a normal cell line system after exposure to 60 Hz of extremely low frequency magnetic field (ELF-MF) and particularly in combination with various external factors, via comet assays. NIH3T3 mouse fibroblast cells, WI-38 human lung fibroblast cells, L132 human lung epithelial cells, and MCF10A human mammary gland epithelial cells were exposed for 4 or 16 h to a 60-Hz, 1 mT uniform magnetic field in the presence or absence of ionizing radiation (IR, 1 Gy), H2O2 (50 μM), or c-Myc oncogenic activation. The results obtained showed no significant differences between the cells exposed to ELF-MF alone and the unexposed cells. Moreover, no synergistic or additive effects were observed after 4 or 16 h of pre-exposure to 1 mT ELF-MF or simultaneous exposure to ELF-MF combined with IR, H2O2, or c-Myc activation.",

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note = "Funding Information: Acknowledgments This work was supported by a Power Generation & Electricity Delivery of the Korea Institute of Energy Technology, Evaluation, and Planning (KITEP) grant (No. 2009101030003E) funded by the Korean Ministry of Trade, Industry & Energy and was also supported by the Ewha Global Top 5 Grant 2012 of Ewha Womans University.",

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doi = "10.1007/s00411-013-0506-5",

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AU - Lee, Ju Woon

AU - Hong, Seung Cheol

AU - Myung, Sung Ho

AU - Lee, Yun Sil

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Absence of DNA damage after 60-Hz electromagnetic field exposure combined with ionizing radiation, hydrogen peroxide, or c-Myc overexpression

Abstract

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Keywords

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