The principal objective of this study was to assess the DNA damage in a normal cell line system after exposure to 60 Hz of extremely low frequency magnetic field (ELF-MF) and particularly in combination with various external factors, via comet assays. NIH3T3 mouse fibroblast cells, WI-38 human lung fibroblast cells, L132 human lung epithelial cells, and MCF10A human mammary gland epithelial cells were exposed for 4 or 16 h to a 60-Hz, 1 mT uniform magnetic field in the presence or absence of ionizing radiation (IR, 1 Gy), H2O2 (50 μM), or c-Myc oncogenic activation. The results obtained showed no significant differences between the cells exposed to ELF-MF alone and the unexposed cells. Moreover, no synergistic or additive effects were observed after 4 or 16 h of pre-exposure to 1 mT ELF-MF or simultaneous exposure to ELF-MF combined with IR, H2O2, or c-Myc activation.
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Acknowledgments This work was supported by a Power Generation & Electricity Delivery of the Korea Institute of Energy Technology, Evaluation, and Planning (KITEP) grant (No. 2009101030003E) funded by the Korean Ministry of Trade, Industry & Energy and was also supported by the Ewha Global Top 5 Grant 2012 of Ewha Womans University.
- Combined treatment
- Comet olive tail moment for DNA damage
- Extremely low frequency