A Visible and Near-Infrared, Dual-Channel Fluorescence-On Probe for Selectively Tracking Mitochondrial Glutathione

Zhiqiang Xu, Xiaoting Huang, Xie Han, Di Wu, Bibo Zhang, Ying Tan, Meijiao Cao, Sheng Hua Liu, Jun Yin, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

136 Scopus citations

Abstract

Glutathione is a major endogenous antioxidant in biological systems. As a result, highly selective fluorescent probes are significant components of studies aimed at gaining a better understanding of the role(s) glutathione plays in biological functions. In this study, we developed a dual-channel turn-on fluorescent probe for glutathione, comprising naphthalimide and cyanine, bridged by a thiol-reactive sulfonamide moiety. The probe selectively responds to glutathione and is not affected by cysteine and homocysteine. Important information has been gained about the chemical mechanism for operation of the probe, which responds to glutathione in visible and near-infrared channels synchronously and independently. Moreover, this probe exhibits the remarkable capability of targeting mitochondria and dual-channel tracking of intracellular glutathione in a spatiotemporal and synchronous manner. We believe that the strategy used to design the new dual-channel fluorescence-on probe will be applicable to the design of fluorescent probes that display specific responses in multiple channels.

Original languageEnglish
Pages (from-to)1609-1628
Number of pages20
JournalChem
Volume4
Issue number7
DOIs
StatePublished - 12 Jul 2018

Keywords

  • SDG3: Good health and well-being
  • cyanine
  • dual-channel fluorescence on
  • fluorescent probe
  • glutathione
  • mitochondria-targeting
  • naphthalimide

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